Study on Rational Breeding of High-Yield Avermectin Industrial Strain  

阿维菌素高产工业菌株的推理选育研究(英文)

作　　者：梁剑光[1,2,3] 储消和 储炬[1] 王永红[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,国家生化工程研究中心,上海200237 [2]常熟理工学院生物与食品工程学院,江苏常熟215500 [3]浙江升华拜克生物股份有限公司,浙江湖州313220

出　　处：《Agricultural Science & Technology》2011年第7期950-953,共4页农业科学与技术（英文版）

基　　金：Supported by 863 Project (2009AA032904 );Scientific ResearchStarting-up Project for Young Teachers in Changshu Institute oTechnology;" Hundreds of Entrepreneurs into Campus" Project inChangshu Institute of Technology~~

摘　　要：[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.[目的]通过推理选育,获得高产的阿维菌素工业生产菌种。[方法]以Biok Av-023为出发菌株,首先采用常规紫外诱变筛选出正突变菌株,再通过添加L-异亮氨酸诱导推理选育方法选育高产阿维菌素生产菌株。[结果]经紫外诱变和L-Ile定向筛选表明,L-Ile筛选浓度以0.5%最佳,经复筛后获得的高产突变菌株AV60s-32最高效价可达4 520 IU/ml,与出发菌株相比提高了23.4%。[结论]采用紫外诱变和L-Ile推理选育相结合的方法可有效提高阿维菌素菌种发酵效价。

关 键 词：AVERMECTIN Strain screening Rational breeding 

分 类 号：TQ927[轻工技术与工程—发酵工程]