bcl-2基因在阻塞性黄疸大鼠肝组织细胞凋亡中作用的研究  

The role of bcl-2 gene in apoptosis of liver in rat with obstructive jaundice

作　　者：王剑明[1] 邹倩[1] 邹声泉[1] 

机构地区：[1]同济医科大学附属同济医院外科,湖北省武汉市430030

出　　处：《世界华人消化杂志》1999年第12期1035-1037,共3页World Chinese Journal of Digestology

摘　　要：目的　探讨阻塞性黄疸大鼠肝组织损害的机制．方法　用末端脱氧核苷酸转移酶（ＴｄＴ） 介导的ｄＵＴＰ 缺口末端标记技术（ＴＵＮＥＬ） 和免疫组织化学方法检测阻塞性黄疸大鼠肝脏组织细胞凋亡状态及凋亡相关基因ｂｃｌ２ 的表达．结果　胆总管结扎后，随结扎时间的延长细胞凋亡增加，结扎１４ ｄ 后细胞凋亡达高峰，凋亡指数（ＡＩ） 达５８－２３ ±１－５８ ，各组ＡＩ差异有显著性意义（ Ｐ＜ ０－０５） ． 在阻塞性黄疸过程中，ｂｃｌ２ 蛋白表达越强，ＡＩ就越少．结论　ｂｃｌ２ 蛋白参与了阻塞性黄疸肝组织中细胞凋亡的调节，并在阻塞性黄疸肝损害的发生和发展中起重要作用．AIM To explore the mechanism of hepatic injury in obstructive jaundice (OJ). METHODS Forty adult male Wistar rats ( 190*!g - 230*!g ) were used in all experiments. All rats underwent laparotomy. Experimental obstructive jaundice (BDL) was induced by double ligation of the bile duct by a proximal ligature around the bile duct in the hilus of the liver and by a distal ligature close to its entry into the duodenum. The animals were randomly divided into two groups: those that underwent bile duct manipulation (sham operated) and those that underwent BDL for 3, 7, 14, and 21 days. We detected the serum bilirubin and pathological change of liver in all rats. Then we detected apoptotic status and the expression of apoptosis related bcl 2 gene protein in liver of all rats with terminal deoxynucleotidyl mediated nick end labeling (TUNEL) and immunohistochemistry method. RESULTS The apoptotic rate of liver is related to times of OJ. Apoptosis was highest 14 days after bile duct ligation, apoptotic index (AI) was 58 23±1 58 ( P <0 05) . The stronger the bcl 2 expression, the less number of apoptotic cells in OJ. The increase of bilirubin and pathological change of liver in OJ were obvious. CONCLUSION bcl 2 takes part in the regulation and occurrence and progression of hepatic injury in OJ.

关 键 词：细胞凋亡 BCL-2基因 阻塞性黄疸 

分 类 号：R575.02[医药卫生—消化系统]