CD28/CD80和CD2/CD58活化PBLs作用肝癌细胞的表型分析与TCR Vβ基因亚家族的优势取用  

Study of the phenotype of PBLs activated by CD28/*!CD80 and CD2/*!CD58 and acting with hepatoma cells and the restricted usage of TCR Vβ gene subfamily

作　　者：肖兰凤 罗利琼 邹奕 黄树林 

机构地区：[1]广东省药学院分子生物学研究室,广东省广州市510224

出　　处：《世界华人消化杂志》1999年第12期1044-1046,共3页World Chinese Journal of Digestology

基　　金：国家自然科学基金;广东省自然科学基金

摘　　要：目的　采用抗ＣＤ２８ ，ＣＤ８０ ，ＣＤ２ 及ＣＤ５８ 分别刺激健康人ＰＢＬｓ 后作用肝癌细胞，对作用前后ＰＢＬｓ 的表型变化及ＴＣＲＶβ基因亚家族的表达水平进行探讨．方法　用ＦＡＣＳ 分析作用肝癌细胞前后ＰＢＬｓ 表型变化，并用ＲＴＰＣＲＳｏｕｔｈｅｒｎ 印迹分析其ＴＣＲ Ｖβ１ ～２０ 的表达水平及特征．结果　健康人ＰＢＬｓ 作用肝癌细胞后ＣＤ３ 和ＣＤ８ 分子表达比作用前明显增高，而ＣＤ４ 分子无显著变化． 健康人ＰＢＬｓ 分别加ＩＬ２ ， ＰＨＡ， 抗 ＣＤ３ 和 ＣＤ３ ＋ ＣＤ２８ ， ＣＤ２８ ＋ ＣＤ８０ ，ＣＤ２ ＋ ＣＤ５８ 作用肝癌细胞（ＢＥＬ７４０２） 前表达水平平均约５ ％ ，作用ＢＥＬ７４０２ 后表达水平约１３ ％ ～２５ ％ ，其特征为Ｖβ７增高．结论　在癌抗原的参与下，ＭｃＡｂ 共刺激的Ｔ 细胞活化，ＴＣＲ接受ＡＰＣ 提呈的相应抗原的刺激，具有该ＴＣＲ 的淋巴细胞迅速增殖而成为针对抗原的Ｔ 细胞克隆，发挥其识别和杀伤癌细胞的作用．AIM To study the difference of the phenotype of PBLs and the expression of TCR Vβ subfamily in liver before and after stimulation by anti CD28, anti CD80, anti CD2 and anti CD58 and then acting with hepatoma cell. METHODS The change in the phenotype of hepatoma cells was studied by RT PCR and Southern blot. RESULTS The level of CD3 and CD8 of PBLs were significantly increased after acting with hepatoma cells, while there was no change in CD4. The mean level of TCR Vβ of PBLs increased from 5% to 13%-25% after acting with hepatoma cell, especially TCR Vβ7 increased dramatically. CONCLUSION With tumor cell antigen, T cells were activated by the stimulation of McAb. The T cells with antigen specific TCR amplified into clone, recognized and killed tumor cells.

关 键 词：McAb共激活 肝癌 膜分子 TCR VΒ基因 

分 类 号：R735.702[医药卫生—肿瘤] R392.11[医药卫生—临床医学]