一种紫贻贝水提多糖的理化性质和结构分析  

Physicochemical properties analyses and structural characterization of a water soluble polysaccharide from Mytilus edulis Linnaeus

作　　者：殷秀红[1] 赵峡[1] 于广利[1] 邢晓旭 张紫恒[1] 

机构地区：[1]海洋药物教育部重点实验室山东省糖科学与糖工程重点实验室中国海洋大学医药学院,山东青岛266003

出　　处：《中国海洋药物》2011年第4期1-6,6,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金(31070724);创新团队发展计划(IRT0944);国家海洋局公益性行业专项(201005024)

摘　　要：目的从紫贻贝中提取和分离纯化多糖,并对其基本理化性质和结构进行分析,为紫贻贝多糖活性研究提供基础。方法将紫贻贝鲜肉制成丙酮粉后,经60℃热水提取,去核酸和采用Sepharcryl S-300凝胶层析分离得到了一种水溶性多糖组分(HWS)。采用硫酸-苯酚法、Folin-酚法、柱前衍生高效液相色谱法和高效凝胶渗透色谱法分别对HWS的总糖含量、蛋白含量、单糖组成、相对分子质量进行了测定,并通过甲基化和气质联用(GC/MS)分析、红外光谱(FT-IR)、核磁共振(NMR)技术对HWS的结构进行了分析。结果 HWS是以(1→4)-α-D-Glcp为主链,含有少量的→2,4)-Glcp-(1→和→6)-β-Glc-(1→分支的葡聚糖,平均每6个主链糖残基含有1个分支。结论采用60℃热水提取和凝胶柱层析分离得到紫贻贝多糖,通过多种化学分析及现代仪器分析技术确定了HWS的结构,为紫贻贝多糖活性的深入研究提供了参考和借鉴。Objective To extract,isolate and purify polysaccharides from Mytilus edulis Linnaeus and determine physicochemical properties and structure of the polysaccharide.Methods A water soluble polysaccaride（HWS） was obtained from acetone powder of Mytilus edulis Linnaeus by water extraction at 60℃,after removing nucleic acid and further purified using Sephacryl S-300 gel-permeation chromatography.The contents of total sugar and protein,the monosaccharide composition,relative molecular mass of HWS were analyzed by phenol-sulfuric method,folin-phenol method,precolumn derivatization high performance liquid chromatography and high performance gel-permeation chromatography,respectively.The structure of HWS was elucidated based on FT-IR and 2D NMR spectroscopy,methylation analysis and GC/MS analysis.Results HWS was a glucan with a（1→4）-linked α-D-Glcp backbone,with a little（1→2,4）-linked-α-D-Glcp and →6）-β-Glc-（1→ side chains.There was about one branching in 6 backbone residues in average.Conclusion The polysaccharide from Mytilus edulis Linnaeus was obtained by water extraction at 60℃ and gel-permeation chromatography.The structure of HWS was confirmed by chemical and modern instruments analyses techniques.Our data will provide basis for the further researches on activities of Mytilus edulis Linnaeus polysaccharides.

关 键 词：紫贻贝 多糖 水提取 理化性质 结构分析 

分 类 号：R913[医药卫生—药学] R917