海洋交替单胞菌YTW-10的鉴定及抑菌活性分析  

Identification and antimicrobial activity analysis of a marine Alteromonas YTW-10

作　　者：王磊[1] 宿红艳[1] 杨润亚[1] 王晓杰[1] 林文杰[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《海洋科学》2011年第7期14-19,共6页Marine Sciences

基　　金：山东省高等学校科技计划项目(J09LC01);山东省优秀中青年科学家科研奖励基金(BS2010SW035)

摘　　要：菌株YTW-10分离自山东烟台海域近海盐场泥样中。该菌能在NaCl浓度为1%～15%的培养基中生长,最适生长NaCl浓度为7%,最适生长温度为37℃,最适pH值为8。经过形态学观察、生理生化特性分析及基于16S rDNA的系统进化分析,该菌株鉴定为交替单胞菌属。以3株水产生物致病细菌和3株农作物致病真菌作为指示菌,检测其抑菌活性。实验结果表明,YTW-10对小麦赤霉病菌、西瓜枯萎病菌和玉米弯胞病菌有明显的抑制效果。The strain YTW-10 was isolated from marine sediments collected from the Yantai beach. It was able to grow in the medium containing 1% - 15% NaCl with the optimal growth salinity of 7%. The optimum growth temperature was 37 ℃ and the initial pH value was 8. Based on morphology, biochemical characteristics, as well as phylogenetic analysis, the strain YTW-10 was identified as Alteromonas. Three marine pathogenic bacteria and three crop pathogenic fungi were used as indicators in YTW-10 antimicrobial experiment. The result showed that YTW-10 had strong antimicrobial activity to Curvlaria lunata, Fusarium ozysporum, and Fusarium graminearum Schw.

关 键 词：交替单胞菌 抑菌活性 鉴定 

分 类 号：Q939[生物学—微生物学]