超声辅助复合酶法提取桑黄多糖  

Extraction technology of polysaccharides from phellinus linteus by ultrasonic-assisted complex enzymatic method

作　　者：尹秀莲[1] 游庆红[1,2] 

机构地区：[1]淮阴工学院生命科学与化学工程学院,江苏淮安223001 [2]南京工业大学食品与轻工学院,江苏南京210009

出　　处：《食品与机械》2011年第4期58-60,共3页Food and Machinery

基　　金：淮阴工学院大学生科技创新重点项目(编号:312409004)

摘　　要：探索超声辅助复合酶法提取桑黄多糖的最佳工艺。以多糖提取收率为指标,对超声时间、复合酶用量、作用时间、酶解温度及pH进行单因素试验研究。结果表明:超声辅助复合酶法提取桑黄多糖的最佳条件为超声时间300s、固定pH 4.0,应用2.0%的木瓜蛋白酶、果胶酶和纤维素酶50℃酶解90min后,多糖得率可达1.46%。该提取工艺多糖提取收率高,可应用于实际生产。Ultrasonic assisted complex enzymatic method was used to optimize the extraction conditions of polysaccharides from phellinus linteus . Using the extraction yield of total polysaccharides as target, the process was optimized by investigating the influence of ultrasonic time, complex enzyme dosage, enzymolysis time, enzymolysis temperature and pH value. Results： Results showed that the optimal extraction conditions for polysaccharides from phellinus linteus by ultrasonic assisted complex enzymatic method were： ultrasonic time 300 s, 2. 0% caroid, 2.0% pectinase plus 2.0% cellulase, extraction temperature 50 ℃, extraction time 90 min and pH 4.0,and in this condition, the polysaccharide yield could reach 1.46 %.

关 键 词：超声 复合酶法 桑黄 多糖 单因素试验 

分 类 号：TQ461[化学工程—制药化工]