HeLa细胞中抑癌基因-间隙连接基因Cx43及其蛋白产物表达缺失  

Expression of antioncogene connexin43 mRNA and its protien in human cervical carcinoma cell line HeLa

作　　者：马向东[1] 陈必良[1] 王德堂[1] 辛晓燕[1] 

机构地区：[1]第四军医大学西京医院妇产科,710033

出　　处：《现代妇产科进展》1999年第4期317-319,共3页Progress in Obstetrics and Gynecology

摘　　要：目的：探讨新一代肿瘤抑制基因-间隙连接基因Cx43及其蛋白产物在人子宫颈癌细胞系HeLa中的表达及意义。方法：应用核酸分子原位杂交和流式细胞仪分析技术，研究人子宫颈癌细胞系HeLa及阳性对照细胞人正常肝细胞系QZG中，间隙连接基因Cx43mRNA及其蛋白产物的表达规律。结果：原位杂交显示，Cx43mRNA在HeLa细胞中呈阴性，在QZG细胞中呈强阳性。流式细胞仪分析示Cx43蛋白在HeLa、QZG细胞中阳性细胞计数率分别为1．9％和99．0％，差异有高度显著性（P＜0．01）。结论：CX43基因及其蛋白产物在HeLa中表达缺失可能与子宫颈癌发生恶性表型相关。Objective To investigate the expression and mechanism of antionco gene connexin (Cx) 43 mRNA and its protein in human cervical carcinoma cell line HeLa.Methods: In situ hybridization(ISH)was employed to detect Cx43 mRNA in HeLa and Cx43 protein was examined by flowcytometer (FCM). Normal liver cell line QZG was used as control. Results: In HeLa cell line, ISH showed that Cx43 mRNA was negative, whereas normal liver cell line QZG was positive. In HeLa and QZG,the positive rates of Cx43 protein were 1. 9% and 99.0% respectively (P<0. 01). Conclusions: The aberrant location of antioncogene Cx43 mRNA and its protein in human cervical carcinoma cell line HeLa may be responsible for genesis of cervical carcinoma.

关 键 词：间隙连接 子宫肿瘤 流式细胞术 CX43基因 表达 

分 类 号：R737.330.2[医药卫生—肿瘤]