低氧条件下大脑皮层微血管平滑肌细胞内皮细胞中[Ca^(2+)]_i变化的研究  

A STUDY ON THE EFFECT OF HYPOXIA ON CALCIUM LEVEL IN CEREBRAL CORTICAL MICROVASCULAR ENDOTHELIAL AND SMOOTH MUSCLE CELLS

作　　者：吕敏[1] 顾正中[1] 

机构地区：[1]中国科学院上海生理研究所,上海200031

出　　处：《中国应用生理学杂志》1999年第4期339-341,共3页Chinese Journal of Applied Physiology

摘　　要：目的和方法：本研究采用离子探针Ｆｕｒａ２／ＡＭ 结合计算机图象分析技术，并通过施加ＮＯ合酶抑制剂ＬＮＮＡ和ＮＯ的作用靶———鸟苷酸环化酶（ＧＣ）的抑制剂美兰（Ｍｅｔｈｙｌｅｎｅ Ｂｌｕｅ；ＭＢ），观察经培养的大鼠大脑皮层微血管内皮细胞和平滑肌细胞中的［Ｃａ２＋］ｉ 在低氧作用后的变化以及与有关血管舒张因子ＮＯ和ｃＧＭＰ之间的关系。结果：低氧时大脑微血管内皮细胞和平滑肌细胞内的Ｃａ２＋ 浓度有所下降，变化幅度的大小与低氧的程度及低氧作用的时间有关，且可以被ＬＮＮＡ和ＭＢ所抑制。结论：低氧时大脑微血管的舒张反应与ＮＯ的产生有关，ＮＯ通过细胞内的多种机制，最终使得胞内Ｃａ２＋Aim and Methods:To study the effect of hypoxia on the change of intracellular calcium level in cultured cortical endothelial cells and smooth muscle cells and to clarify whether nitric oxide (NO)and cGMP participates in the hypoxic cerebral vasodilatory response. Results:The intracellular calcium level in endothelial cells and smooth muscle cells were decreased under hypoxia, and the magnitudes of change were enhanced by increasing the degee and duration ot hypoxia. The hypoxic descent of [Ca 2+ ]i level in these two kinds of cells could be inhibited by L NNA and MB.Conclusion:There exists a relation to NO and cGMP.The hypoxic cerebrovascular dilation was likely the result of the increased NO concentration which then caused the decreased of [Ca 2+ ] i through some pathways.

关 键 词：低氧 微血管 平滑肌细胞 内皮细胞 脑缺氧 钙 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R364.4[医药卫生—临床医学]