ADAM17-siRNA对MCF-7人乳腺癌细胞增殖的影响  

Effects of ADAM17-siRNA on Proliferation of Human MCF-7 Breast Cancer

作　　者：路延芹[1] 赵光明[2] 张雪鹏[2] 

机构地区：[1]河北联合大学,河北唐山063000 [2]河北联合大学附属医院,河北唐山063000

出　　处：《现代中西医结合杂志》2011年第24期3003-3005,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的利用RNAi使解聚素-金属蛋白酶17(ADAM17)基因沉默,观察AD-AM17 mRNA在人MCF-7乳腺癌细胞中的表达及其对细胞增殖的影响。方法利用脂质体介导ADAM17-siRNA转染MCF-7细胞,采用实时荧光聚合酶链反应(Real-time PCR)检测ADAM17 mRNA表达的水平;MTT法检测细胞增殖的活性;流式细胞仪检测细胞周期。结果 ADAM17 mRNA在MCF-7细胞中高表达,转染特异性ADAM17-siRNA后,其表达显著被抑制,同时MCF-7细胞增殖能力也明显下降,细胞进入S和G2/M期的比例降低,绝大多数细胞停留在G0/G1期。结论 ADAM17-siRNA可通过脂质体有效转染MCF-7人乳腺癌细胞,转染后的MCF-7细胞增殖能力明显下降,这种作用与阻止癌细胞进入增殖周期有关。Objective It is to investigate the effect of ADAM17-siRNA on the proliferation of human MCF-7 breast cancer cell by silence ADAM17 gene with siRNA.Methods ADAM17-siRNA was transfected into MCF-7 cells mediated by oligofectamine.ADAM17 mRNA expression were detected by Real-time PCR;the proliferative ability and cell cycle change of MCF-7 cells were detected by MTT assay and flow cytometry respectively.Results ADAM17 mRNA had the high expression in MCF-7 cells,while it significantly decreased with ADAM17 siRNA treatment.At the same time,ADAM17-siRNA markedly inhibited the proliferation of MCF-7,associated with preventing cells into proliferating cycle.Conclusion ADAM17-siRNA is transfected efficiently mediated by oligofectamine,which leads to decrease the proliferative ability and prevent cells into proliferating cycle.

关 键 词：解聚素-金属蛋白酶17 乳腺癌 RNA干扰 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]