牛磺酸对局灶性脑缺血大鼠神经干细胞因子mRNA表达的影响  

Effects of taurine on the expression of neurons SCF mRNA of focal cerebral ischemia in rat

作　　者：臧艳平[1] 梁桂霞[2] 高焕民[3] 

机构地区：[1]山东省青岛市第八人民医院神经科,266042 [2]宁夏回族自治区人民医院查体中心,750021 [3]宁夏回族自治区人民医院神经科,750021

出　　处：《国际中医中药杂志》2011年第8期689-691,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的观察牛磺醪对大鼠缺血性脑损伤后神经干细胞因子mRNA表达的影响。方法成年雄性SD大鼠63只，采用线栓法建立右侧大脑中动脉缺血再灌注模型，缺血时间为2h后再灌注。应用随机数字表进行完全随机化的分组，分为治疗组（自造模当日开始，静脉注射牛磺酸80mg／kg体重，持续10min，1次／d，连用7d）和对照组（静脉注射0．9％氯化钠注射液1ml，其余相同）各30只，每组再按同样的随机方法分为再灌注2h、24h、3d、7d、14d组（每组6只），另取3只为假手术组。应用原位杂交检测脑缺血再灌注后神经干细胞因子（stem cell factor，SCF）mRNA的表达。结果假手术组SCFmRNA在皮质、纹状体和室旁区有微弱表达。对照组缺血侧SCFmRNA的表达，皮质除2h以外、纹状体除2h以外、室旁区除2h、14d以外各时间点均高于假手术组（t=2．16～25．19，P〈0．05）。治疗组SCFmRNA表达较对照组在皮质、纹状体及室旁区均显著升高，（t=5．19-26．17，P〈0．05）。结论牛磺酸可促进大鼠局灶性脑缺血再灌注后SCFmRNA的表达。Objective To investigate the effects of taurine on the expression of stem cell factor mR.NA of the focal cerebral ischemia in rat. Methods 63 adult male rats were randomly divided into taurine-treated group （Taurine, 80 mg/kg, began at the day of MCAO and continuously intravenous injection for 10 min, daily for 7 days） and controlled group （0.9% normal saline, 1 ml, continuously intravenous injection for 10 min daily for 7 days）. The rats received right middle cerebral artery occlusion at 2h and different hours of reperfusion. Expression of SCF was detected by in situ hybridization in the rats subjected to 2 h, 24 h, 3 d, 7 d, 14 d of reperfusion and sham-operated group （n= 3）. Results Low level SCF mRNA expressions were found in cortex, striatum and extraventricular zone in sham-operated group. The expression of SCF mRNA in ischemic hemisphere of controlled group increased markedly compared with sham-operated group （t= 2.16-25.19, P〈 0.05）, besides that of 2 h in cortex, 2 h in striatum, 2h and 14d in extraventricular zone. SCF mRNA expression in the treatment group increased markedly in cortex, striatum and extraventricular zone at 3d and 7d （t= 5.19- 26.17, P〈0.05）. Conclusion Taurine increased the SCF mRNA expression in MCAO rats, therefore it might promote the creation of neural stem cell.

关 键 词：牛磺酸 神经元 干细胞因子 缺血再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]