犬新孢子虫吉林株SAG1基因在BHK21细胞中的表达及免疫学评价  

Transient expression of Neospora caninum Jilin strain SAG1 gene in BHK21 cells and the evaluation of immunology

作　　者：张守发[1] 栾杨[1] 贾立军[1] 鞠玉琳[1] 鲁承[1] 张西臣[2] 

机构地区：[1]延边大学农学院动物医学系,吉林龙井133400 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2011年第8期1166-1168,1174,共4页Chinese Journal of Veterinary Science

摘　　要：将构建成功的重组质粒pVAX-SAG1转染至BHK21细胞上,通过IFAT与RT-PCR方法检测重组质粒在BHK21细胞上的表达情况。大量提取重组质粒pVAX-SAG1,免疫Balb/c小鼠,分别在首免前、二免前、三免前以及三免后的第1、2、3周进行小鼠断尾采血,应用ELISA方法与流式细胞技术检测体液免疫水平和细胞免疫水平。结果表明,转染的重组质粒pVAX-SAG1在BHK21细胞中得到瞬时表达,并且重组质粒pVAX-SAG1免疫Balb/c小鼠后,在体液免疫水平上,pVAX-SAG1组与空载体pVAX1和PBS对照组差异极显著(P<0.01);在细胞免疫水平上,pVAX-SAG1组与PBS对照组差异显著(P<0.05)。本试验为新孢子虫基因工程核酸疫苗的进一步研究奠定了基础。The correct recombinant plasmid pVAX-SAG1 was transfected into BHK21 cells.The expression condition of the recombinant plasmid was detected by IFAT and RT-PCR.A great quantity of the recombinant plasmid pVAX-SAG1 was extracted.Balb/c mice were immunized by pVAX-SAG1.Mice＇s blood was got by cutting their tails before the first immunity,the second immunity,the third immunity and on the first week,the second week,on the third week after the third immunity.The result indicated that that the recombinant plasmid was transiently expressed.After Balb/c mice were immunized by pVAX-SAG1,in the humoral immune level,pVAX-SAG1 group and empty vector pVAX1、PBS control group was significantly different（P0.01）;in the cellular immunity level,pVAX-SAG1 group and PBS control group was significantly different（P0.05）.This research settled a solid foundation for preparing the genetically engineering vaccine against Neospora caninum.

关 键 词：犬新孢子虫 SAG1基因 真核表达 免疫水平 

分 类 号：S852.7[农业科学—基础兽医学]