类人源猪H3N2亚型流感病毒HA,NA遗传进化分析  

Phylogenetic analyses of the HA and NA genes of human-like H3N2 subtype swine influenza viruses

作　　者：樊娇[1] 李鑫[1] 张泉鹏[1] 王广美[1] 丛彦龙[1] 丁壮[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2011年第8期1175-1179,1188,共6页Chinese Journal of Veterinary Science

基　　金：吉林大学农学部引进人才科研启动基金资助项目(4305050102B6)

摘　　要：2007-2008年从吉林省某猪场采集疑似流感发病猪的鼻咽拭子,经病毒分离鉴定获得3株H3N2亚型流感病毒,分别命名为A/swine/Jilin/5/2007(Sw/Jilin/5/07)、A/swine/Jilin/19/2007(Sw/Jilin/19/07)、A/swine/Jilin/37/2008(Sw/Jilin/37/08)。HA进化树分析结果表明:3株H3N2亚型流感病毒属于近代人源病毒谱系;但是在NA进化树中,Sw/Jilin/37/08株与早期人源和近代人源关系密切,暗示它可能是早期人源和近代人源H3N2亚型猪流感病毒之间的过渡毒株,进一步证实猪在流感病毒种间传播过程中充当"中间宿主"作用。Three strains of H3N2 subtype swine influenza viruses from nasopharyngeal swabs of pigs with influenza-like signs in Jilin province from 2007 to 2008 were isolated and identified by RT-PCR and HI methods.Phylogenetic analyses of HAs showed that the HAs of three isolates tested in this study were the contemporary human virus origin,while in the NA tree,Sw/Jilin/37/08 was equally closely related to the early and contemporary H3 human strains as well as an intermediate virus between the early human lineage and the contemporary human lineage.These results were further to confirm the role of intermediate host of pigs in the interspecies transmission of influenza viruses.It emphasizes the importance of reinforcing swine influenza virus surveillance in China.

关 键 词：猪流感病毒 H3N2 遗传分析 

分 类 号：S852.65[农业科学—基础兽医学]