川牛膝种子发芽试验与生活力测定方法的研究  

Study on Method of Seed Germination Testing and Viability Assay of Cyathula officinalis Kuan.

作　　者：刘千[1] 罗浩[1] 蔡文国[1] 吴卫[1] 

机构地区：[1]四川农业大学农学院,成都625014

出　　处：《种子》2011年第7期20-22,25,共4页Seed

基　　金：国家重大科技专项"中药材种子种苗和种植(养殖)标准平台"(2009ZX09308-002)子课题(047)资助

摘　　要：针对目前尚无川牛膝种子发芽和生活力检测标准的现状,采用不同的发芽床、发芽温度和前处理方法进行发芽试验;采用红墨水法、BTB法和TTC法对川牛膝种子生活力进行测定。结果表明,褶裥纸、25℃和不作前处理为最适宜的发芽条件;第2天和第9天分别为初末次计数时间;浓度1%的TTC溶液染色3 h为最佳生活力测定方法。Considering the lack of seed germination testing and viability assay standards for Cyathula officinalis Kuan.,this paper studied the effects of different germination beds,temperatures and pre-treatments on seed germination.Red ink staining,BTB staining and TTC staining were used for determining seed viability assay.The results showed that pleated paper,25 ℃ and no pre-treatment were the optimum conditions for seed germination.The 2 nd day and the 9 th day were the first and the last count time.The 1% TTC solution dyed 3 h was the best method for seed viability assay of Cyathula officinalis Kuan.

关 键 词：川牛膝 种子 发芽 生活力 

分 类 号：Q949.95[生物学—植物学]