转化生长因子-β通过PI3K／AKT信号通路促进人肺腺癌细胞株A549的增殖作用  

Effect of transforming growth factor-β in human lung cancer cell line A549 on on cell proliferation and its mechanism

作　　者：胡伟[1] 胡祎[2] 周昆[1] 王跃斌[1] 夏宗江[1] 

机构地区：[1]郑州大学第一附属医院胸外科,450052 [2]中山大学肿瘤防治中心胸科

出　　处：《中华实验外科杂志》2011年第8期1241-1243,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨转化生长因子-β（TGF-β）在人肺癌细胞株A549中对细胞增殖的影响及其机制。方法通过抑制组50例和促进组50例两个方面验证TGF-β对肺癌细胞株A549的促增殖作用，噻唑蓝（MTT）比色法测定细胞增殖速率，流式细胞仪检测细胞的早晚期凋亡和细胞增殖状态。结果经过siTGF-βR2处理的细胞，细胞增殖率为（91．8±2．3）％，增殖能力明显减弱；而经过mTGF-β处理的细胞，细胞增殖率为（112．8±5．6）％，增殖能力则明显增强。流式细胞仪结果显示各组的凋亡率分别为：空白组（6．81±0．11）％，随机干扰siRNA组为（6．75±0．12）％，rhTGF-β1组为（6．10±0．10）％，siTGF-βR2组为（7．77±0．09）％，LY294002组为（7．82±0．14）％。结论TGF-β通过P13K／AKT信号通路促进人肺腺癌细胞株A549的增殖。Objective To investigate the effect of transforming growth factor （TGF）-β in human lung cancer cell line A549 on cell proliferation and its mechanism. Methods TGF-β promoted proliferation of human lung cancer cell line A549 （50 cases）, which was validated both in respect of inhibition and promotion. Cell proliferation rate was determined by methyl thiazol tetrazolium （MTr）. The apotosis and proliferation were analyzed by flow cytometry in early and later stages of cells. Results Cell proliferation rate of A549 cells was （91.8 ＋ 2. 3 ）% , which was significantly decreased after treatment with siTGF-βR2. On the contrary, the rate was （112. 8 ＋ 5.6）%, which was significantly enhanced after treatment with rhTGF-β. Flow cytometry demonstrted that apotosis rate in control group, random interference siRNA group, rhTGF-β1 group, siTGF-βR2 group and LY294002 group was （6. 81 ＋ 0. 11 ）%, （6.75 ＋ 0.12）%, （6.10＋0.10）%, （7.77＋0.09）%, and （7.82＋0.14）% respectively. Conclusion TGF-β promotes proliferation of human lung adenocarcinoma cell line A549 through the PI3K/AKT pathway.

关 键 词：转化生长因子-Β 肺癌 增殖 脱噬作用 

分 类 号：R734.2[医药卫生—肿瘤]