半乳糖凝集素1在CD133^＋肺腺癌细胞中的表达和功能  

Expression and function of Galectin-1 in CD133^＋ human pulmonary adenocarcinoma cells

作　　者：周雪峰[1] 但攀[1] 张力[1] 朱少平[1] 赵金平[1] 

机构地区：[1]武汉大学中南医院胸心外科,430071

出　　处：《中华实验外科杂志》2011年第8期1244-1246,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：中央高校基本科研业务费专项资金资助项目（111118）;湖北省卫生厅年度科研资助项目（NX2011-6）

摘　　要：目的观察肺腺癌CD133^＋细胞中半乳糖凝集素1（Galeetin-1）的表达和功能。方法磁珠分选出9例患者肺腺癌中CD133^＋细胞并以流式细胞术检测分选效率，荧光实时定量聚合酶链反应（fqRT—PCR）、Westernblot和酶联免疫吸附试验（ELISA）检测CD133^＋细胞中或上清液中Galectin-1的表达，Galeetin-1小干扰RNA（siRNA）转染CD133^＋细胞后检测其对肿瘤细胞生长以及克隆形成能力的影响。予裸鼠皮下注射Galeetin-1 siRNA转染后的CD133^＋细胞并观察肿瘤的生长。结果流式细胞术结果表明磁珠分选出的细胞中CD133^＋细胞率为92．6％。fqRT—PCR和Westemblot检测结果显示Galectin-1在CD133^＋细胞中的表达量分别是CD133-细胞中的1．748倍和1．135倍。体外显示发现下调CD133^＋细胞中Galeetin．1表达后肿瘤细胞的增殖率为（36．75±1．35）％，对照组为（92．31±0．78）％，差异有统计学意义（P〈0．05）。结论Galeetin-1在肺腺癌干细胞中高表达，并能促进肺腺癌干细胞的生长增殖。Objective To investigate the expression and function of Galectin-1 in CD133^± pulmo- nary adenocarcinoma cells. Methods CD133^± ceils were separated by magnetic activated cell sorting （MACS） from excised pulmonary adenocarcinoma speciments of 9 patiens. The proportion of CD133^± cells was measured by flow cytometry （FCM）. The expression of Galectin-1 in CD133^± or CD133^- cells was quantitated by fluorescent quantitation real-time polymerase chain reaction （fqRT-PCR）, Western blotting and enzyme linked immunosorbent assay （ELISA） respectively. CD133^± cells were transfected with small interfering RNA （siRNA） of Galectin-1 to study the effect of Galectin-1 inhibition on cancer cells growth and clonality. Tumorigenesis in nude mice was also performed in vivo. Results 92. 6% cells separated by MACS were positive for CD133, which was proved by FCM. The expression of Galectin-1 in CD133^± cells was 1. 748 folds and 1. 135 folds higher than that in CD133^ - cells detected by fqRT-PCR and Western blot respectively. Downregulation of Galectin-1 ex vivo also resulted in （36. 75 ± 1.35） % decrease of proliferation rate of cancer cells. Conclusion Galectin-1 which can be efficiently inhibited by Galectin-1 siRNA was significantly highly expressed in CD133^＋ cells and associated with the proliferation and clonality of CD133^＋ cells.

关 键 词：半乳糖凝集素 CD133 肺腺癌 

分 类 号：R734.2[医药卫生—肿瘤]