细胞分裂周期蛋白42mRNA和蛋白在食管鳞癌中的表达及其病理生物学意义  

Expression of cell division cycle 42 and its clinicopathological significance in esophageal squamous cell carcinoma

作　　者：冯军国[1] 郑树涛[1] 刘辉[1] 刘涛[1] 林仁勇[1] 卢晓梅 

机构地区：[1]新疆医科大学第一附属医院中心实验室,乌鲁木齐830054 [2]新疆维吾尔自治区食管癌研究所

出　　处：《中华实验外科杂志》2011年第8期1258-1260,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30860097）

摘　　要：目的探讨细胞分裂周期蛋白42（Cde42）在食管鳞状细胞癌（ESCC）中的表达及其与临床病理参数间的关系。方法应用实时荧光定量．聚合酶链反应（qRT—PCR）、蛋白免疫印迹和免疫组织化学方法，从mRNA和蛋白两个水平检测ESCC中Cde42的表达，并分析其与临床病理参、数的关系。结果22对新鲜ESCC与癌旁正常组织中，Cde42mRNA在ESCC中的表达量（0．21±0．14）显著高于其在癌旁正常组织中（0．16±0．12）的表达（P〈0．05）；Cde42蛋白在ESCC中的表达、量（0．83±0．35）高于其在癌旁正常组织中（0．75±0．24）的表达；在175对ESCC中，Cdc42蛋白的阳性表达率为73．7％（129／175），高于其在配对的癌旁正常组织中的表达62．9％（110／175，P〈0．05）。此外，Cde42的表达与ESCC患者的年龄、淋巴结转移及分化程度明显相关（P〈0．05）。结论Cdc42可能参与ESCC发生及转移的过程。Objective To explore the expression of cell division cycle 42 （ Cdc42 ） in human esophageal squamous cell carcinoma （ESCC） and investigate the association between Cdc42 and clinicopathological parameters. Methods The expression levels of Cdc42 mRNA and protein in ESCC and corresponding adjacent normal tissues were detected by real-time fluorescent quantitative polymerase chain reaction （qRT-PCR）, Western blotting and immunohistochemistry, respectively. The correlations between Cdc42 expression and clinicopathological parameters were analyzed. Results The expression of Cdc42 mRNA was significantly higher in ESCC tissues （0. 21 ± 0. 14 ） than that in corresponding controls （0. 16 ±0. 12） （t test,P 〈0. 05）. The protein expression of Cdc42 was significantly higher in ESCC tissues （0. 83 ± 0. 35 ） than that in corresponding controls （0. 75 ± 0. 24 ）. Immunohistochemistry revealed that 73.7% （129/175） of the ESCC samples had higher expression of Cde42 protein than the corresponding controls [62. 9% （110/175） （2＂2 test, P 〈 0. 05 ）]. Cdc42 expression level was correlated with age, lymphoid node metastasis and differentiation （P all 〈 0. 05 ）, but not with the elinicopathological features, such as gender, ethnicity and macroscopical types （ P 〉 0. 05 ）. Conclusion The higher expression of Cdc42 played a certain role in the carcinogenesis and metastasis of ESCC.

关 键 词：食管鳞状细胞癌 细胞周期 侵袭 转移 

分 类 号：R735.1[医药卫生—肿瘤]