Cyclin E和p27^kip1在不同时段增生性瘢痕的表达及与细胞周期的关系  

Expression of Cyclin E and p27^kip1 in the different stages of hypertrophic scar and its correlation with fibroblastic cell cycle

作　　者：唐红梅[1] 王达利[1] 张学军[2] 金文虎[1] 邵蔚[1] 邹安芳[1] 

机构地区：[1]遵义医学院附属医院烧伤整形外科,贵州564300 [2]遵义医学院附属医院神经外科,贵州564300

出　　处：《中华实验外科杂志》2011年第8期1283-1285,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：贵州省科技攻关计划资助项目[黔科合S字（2007）1040]

摘　　要：目的探讨不同时段增生性瘢痕（HS）成纤维细胞（FB）细胞周期相关基因Cyclin E和p27^kip1基因及蛋白的表达与FB实际所处的细胞周期的关系。方法手术切取32例HS4组：3个月、6个月、1年及2年组，应用逆转录一聚合酶链反应（RT—PCR）技术，观察不同阶段HS中Cyclin E和p27^kip1的mRNA表达，用Western blot观察两者的蛋白表达水平，流式细胞术检测各个阶段HS中FB的细胞周期。结果（1）随着HS的发展，FB中CyclinE的mRNA和蛋白的表达强度呈由强至弱的变化；而p27^kip1 mRNA和蛋白的表达早期明显低于晚期。3个月组与6个月组中Cyclin E的mRNA表达差异有统计学意义（P〈0．05）；CyclinE和p27^kip1在3个月组与6个月组中的蛋白表达差异有统计学意义（P〈0．05），在3个月组及6个月组分别与1年组、2年组mRNA和蛋白的表达差异均有统计学意义（P〈0．05）。（2）流式细胞仪检测结果：3个月组、6个月组HS的FB多数处于s期；而1年及2年组的HS的FB多处于G0／G1期。结论不同时期增生性瘢痕中CyclinE和p27^kip1 mRNA和蛋白的表达趋势基本一致，而且其细胞成分的细胞周期分布与这两个蛋白所执行的功能情况相对应；因此在HS发生早期对这两个基因进行干预，有可能会较好控制HS的发生及发展。Objective To investigate the correlation of the mRNA and protein expression of Cyclin E and p27^kip1 in different stages of hyperplastic scar with fibroblastic cell cycle. Methods Thirty-two samples of hyperplastic scar were divided into four groups according to the developing stages of hyperplastic scar： 3 months group, 6 months group, 1 year group and 2 years group. Reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting staining methods were used to detect the mRNA and protein expression of Cyclin E and p27^kip1 respectively in fibroblasts of the hypertrophic scar. By using flow cytometry, the fibroblastic cell cycle was assayed. Results （1） With the development of hypertrophic scar, the expression intensity of Cyclin E in fibroblasts experienced from high level to low level, and the expression of p27^kip1 in early stages of hypertrophic scar was evidently lower than in advanced stages of hypertrophic scar. There was significant difference between 3 months group and 6 months group in the expression of Cyclin E mRNA （P 〈0. 05） and in the expression of Cyclin E and p27^kip1 plproteins （P 〈0. 05）, and between 3 months group or 6 months group and 1 year group or 2 years group in the expression of Cyclin E and p27^kip1 mRNA and proteins （P 〈 0. 05） ; （2） Flow cytometry revealed that most of hypertrophic scar fibroblasts in 3 months group and 6 months group were at S phase, and those in 1 years and 2 years groups at G0/G1 phase. Conclusion The mRNA and protein expression levels of Cyclin E and p27^kip1 are basically identi- cal in different stages of hypertrophic scar. The distribution of cell cycle also accords with the functions of Cyclin E and p27^kip1 in different stages of hypertrophic scar. An early stage intervention to the two genes can effectively prevent the genesis and development of hypertrophic scar.

关 键 词：增生性瘢痕 CYCLIN E 细胞周期 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]