双特异性单链抗体介导的CIK细胞对结肠癌的体内杀伤作用  

Killing effect of CIK cell mediated by bispecific single-chain antibody in vivo on colon cancer

作　　者：刘洪一[1] 李荣[2] 刘巨超[2] 李力[2] 梁文涛[2] 徐迎新[2] 

机构地区：[1]解放军总医院肿瘤外科,北京100853 [2]解放军总医院普通研究所,北京100853

出　　处：《中华实验外科杂志》2011年第8期1342-1343,共2页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家高技术研究发展计划资助项目（2006AA022489）

摘　　要：目的观察双特异性单链抗体介导的细胞因子诱导的杀伤细胞（CIK）细胞在体内杀伤结肠癌细胞的作用。方法将SWlll6细胞种植裸鼠后，建立裸鼠结肠癌模型，分为3组分别经尾静脉注入生理盐水、CIK细胞及CIK细胞＋双特异性抗体，每周治疗1次，共4周，取出肿瘤组织称重，并计算出各组的抑瘤率。结果在体内，CIK组和CIK＋双抗组均可抑制肿瘤生长，抑瘤率分别为29．70％和65．02％，与对照组比较差异均有统计学意义（P〈0．05）。CIK组和CIK＋双抗组的瘤体积、瘤重、抑瘤率比较，差异均有统计学意义（P〈0．05）。结论CIK细胞本身可以抑制肿瘤细胞的生长，在加入抗CD3抗癌胚抗原（CEA）双特异性单链抗体后抑瘤作用明显增强。Objective To investigate the killing effect of cytokine-indueed killer cell （CIK） me- diated by bispecific single-chain antibody in vivo on colon cancer cells. Methods The colon cancer model in nude mice was established with SW1116 cells. The mice were divided into 3 groups, which were injected with normal saline, CIK cells and CIK cells ＋ bispecific antibody, respectively, once a week for 4 weeks. The tumors were removed and weighed, and the tumore inhibition rate in each group was calculated. Results The CIK cells and CIK cells with bispecific single-chain antibody could both inhibit tumor growth in vivowith the tumor inhibition rate being 29. 70% and 65.02% respectively （ P 〈 0. 05 ）. There was significant difference in tumor inhibition rate between CIK cells and CIK cells with bispecific singlechain antibody （ P 〈 0. 05 ）. Conclusion CIK cells alone can inhibit tumor cell growth, CIK cells in combination with bispecific single-chain antibody can exert more significant antitumor effect.

关 键 词：结肠癌 CIK细胞 双特异性单链抗体 

分 类 号：R733.71[医药卫生—肿瘤]