血管内皮生长因子C在肝癌组织的表达及其意义  

The expression and significance of vascular endothelial growth factor C in hepatocellular carcinoma

作　　者：吕海涛[1] 刘三光[1] 穆宏凌[3] 刘润田[1] 刘建华[1] 高翔[2] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北医科大学第二医院血管外科,石家庄050000 [3]河北省卫生厅考试培训中心

出　　处：《中华实验外科杂志》2011年第8期1387-1389,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测血管内皮生长因子（VEGF）-C在肝癌组织中的表达，探讨肝癌生长过程中存在未折叠蛋白反应（UPR）的激活。方法采用逆转录一聚合酶链反应（RT—PCR）检测新鲜的42例肝癌标本、15例同个体癌旁1．0cm肝组织和15例正常肝组织中VEGF．C的mRNA表达，经Gel—proAnalyzer3．1软件分析RT-PCR产物的吸光度（A），计算VEGF—C的A值与内参照基因（GAPDH）A值的比值，作为目的基因产物mRNA的相对含量。结果肝癌组织、癌旁肝组织、正常肝组织中VEGF—CmRNA相对表达量分别为0．4447±0．0335、0．4195±0．0334、0．4019±0．0259，VEGF—C mRNA在3组之间的表达呈下降趋势（P〈0．05），在肝癌组织和正常肝组织中均有VEGF—C mRNA的表达，但肝癌组织中的表达明显高于正常肝组织。结论在肝癌的生长过程中存在未折叠蛋白反应的激活。在肝癌组织中VEGF—CmRNA呈高表达。在肝癌的发生过程中，未折叠蛋白反应可能有重要作用。Objective To detect the express of vascular endothelial growth factor （VEGF）-C mRNA in the tissue of hepatocellular carcinoma ; demonstrating that it exist activation of unfold protein response （UPR） in process of growth, and the expression of UPR was higher than normal tissue. Methods To study the mRNA of VEGF-C in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse transcription-polymerase chain reaction （RT- PCR）. The 42 case example of hepatome was all diagnosed by pathology. By the software of Gel-proAnalyzer 3. 1, analyzing the optical density （A） of the production of RT-PCR. And calculusing the ratios of the A value between VEGF-C and glyeeraldehydes-3-phosphate dehydrogenase （GAPDH） to represent the relative amount of view gene product. Results The express of VEGF-C mRNA in hepatoma tissue adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4447 ± 0. 0335, 0. 4195 ± 0. 0334, 0. 4019 ±0. 0259. The express of VEGF-C mRNA was higher in hepatoma than others （ P 〈 0. 05）. The experiment indicate that the VEGF-C express in the hepatoma tissue and normal hepatic tis- sue, but it was higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist activation of UPR in process of growth. The express of VEGF-C mRNA was accordant. It was higher in hepatoma tissue than in normal hepatic tissue. The changes of its gene is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma. It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis.

关 键 词：肝癌 肝组织 血管内皮生长因子C RT—PCR 

分 类 号：R735.7[医药卫生—肿瘤]