台湾甜椒种子中辣椒轻斑驳病毒检测及其致病型鉴定  

Detection and Pathotype Identification of Pepper Mild Mottle Virus in Sweet Pepper Seeds from Taiwan

作　　者：廖富荣[1] 沈建国[2] 吴媛[1] 郭木金[1,3] 黄蓬英[1] 陈青[1] 陈红运[1] 林石明[1] 

机构地区：[1]厦门出入境检验检疫局检验检疫技术中心,福建厦门361026 [2]福建出入境检验检疫局检验检疫技术中心,福建福州350003 [3]福建农林大学,福建福州350002

出　　处：《热带作物学报》2011年第6期1128-1135,共8页Chinese Journal of Tropical Crops

基　　金：质检公益性行业科研专项项目(No.201010256);厦门出入境检验检疫局科技计划项目(No.2010XK05);福建省自然科学基金计划资助项目(No.2009J01046);国家质检总局科研项目(No.2010IK272)

摘　　要：利用双抗体夹心酶联免疫吸附法(DAS-ELISA)、反转录聚合酶链式反应(RT-PCR)和基因序列分析法对一批台湾进境的辣椒种子进行病毒检测及致病型鉴定,同时使用特征序列扩增区域(SCAR)标记引物利用PCR方法对该辣椒品种进行L抗性基因检测。DAS-ELISA及RT-PCR结果表明,从该批种子中检测到辣椒轻斑驳病毒(Peppe rmild mottle virus,PM MoV)。PCR产物测序分析表明,该序列为PM MoV的外壳蛋白(CP)基因,与已报道的PM MoV序列同源性为92.4%～99.8%。CP蛋白氨基酸序列分析表明,该分离物(命名为TW分离物)属于PM MoV的P1,2,3致病型。抗性基因检测表明,该辣椒品种携带有L3抗性基因。TW分离物能够系统侵染该辣椒品种,意味着该分离物已经克服L3基因介导的抗性。本研究报道的PM MoV的P1,2,3致病型在中国大陆尚属首次,这对于抗病辣椒品种的选育具有重要的指导意义。The seed-transmitted viruses were detected in sweet pepper （Capsicum annuum L.） seeds from Taiwan, using double antibody sandwich-enzyme linked immunosorbent assay （DAS-ELISA）, reverse transeription-polymerase chain reaction （RT-PCR） and sequencing, and the sweet pepper cuhivar L resistance gene was detected by PCR using sequence characterized amplified region （SCAR） marker prime~. The results of DAS-ELISA and RT-PCR showed that Pepper mild mottle viru~ （PMMoV） were found in the seeds. Sequence determination results proved that the fragment was composed of coat protein of PMMoV, sharing identities of 92.4%-99.8% with other PMMoV isolates at nucleotide level. The amino acid sequence analysis showed that this isolate （disignate TW isolte） belonged to P12,3 pathotype of PMMoV. The detection result of L resistance gene showed that this sweet pepper cuhivar possessed L3 resistance gene. The TW isolate was able to systemically infect this sweet pepper cultiva： and suggested that it had overcome L3 gene-mediated resistance. This is the first report of Pt2,3 pathotype of PMMoV in China and it would have guiding significance in pepper resistance breeding.

关 键 词：甜椒 辣椒轻斑驳病毒 种子传播 外壳蛋白基因 P1 2 3致病型 L3抗性基因 

分 类 号：S641.3[农业科学—蔬菜学]