农业杀虫剂敌百虫对杜氏盐藻的毒性作用  

Toxic Effects of Chemical Pesticide Trichlorfon on Dunaliella salina

作　　者：范一文[1] 陈辉[2] 姜建国[1,2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2011年第8期877-880,共4页Modern Food Science and Technology

基　　金：2010年大学生创新基地建设项目

摘　　要：本文以一种具有环境耐性的单细胞绿藻杜氏盐藻（Dunaliella salina）作为受试生物,检测杀虫剂敌百虫的毒性作用。实验中检测了敌百虫对盐藻细胞生长、β胡萝卜素水平及超氧化物歧化酶（Superoxide dismutase,SOD）和过氧化氢酶（Catalase,CAT）活性的影响。当敌百虫浓度小于0.050 g/L时,细胞的毒性响应与对照样类似。当使用0.075~0.100 g/L敌百虫处理盐藻,处理初期细胞生长和β胡萝卜素水平降低,一段时间后恢复。当敌百虫浓度大于0.125 g/L,细胞生长和β胡萝卜素水平都会一直降低直至检测不到。敌百虫对杜氏盐藻的10-d IC50是0.179 g/L。实验发现在低浓度下敌百虫会刺激CAT活性的增长。Dunaliella salina,a unicellular green alga of environmental tolerance,was employed as test organism to investigate the toxicity effects of a pesticide trichlorfon.The influences of trichlorfon on cell growth,β-carotene level and activities of superoxide dismutase（SOD） and catalase（CAT） were investigated.At the concentrations less than 0.050 g/L trichlorfon,cell responses were similar to that of the control.When treated with 0.075~0.100 g/L trichlorfon,cell growth and β-carotene levels declined at first and then revived.When concentrations were higher than 0.125 g/L trichlorfon,both cell growth and β-carotene levels decreased until they were undetectable.The 10-d IC50 of trichlorfon on D.salina was 0.179 g/L.Trichlorfon could stimulate the increase of CAT activity at a low concentration.

关 键 词：杜氏盐藻 毒性 敌百虫 

分 类 号：S482.3[农业科学—农药学] Q945[农业科学—植物保护]