超临界CO_2萃取小球藻精油及其抗氧化分析  

Antioxidant Activity of Chlorella Essential Oil Extracted by Supercritical CO_2 Fluid

作　　者：厉剑剑[1] 张文焕[1] 黄惠华[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2011年第8期938-941,共4页Modern Food Science and Technology

基　　金：国家十一五科技支撑计划项目(2006BAD27B03);广东省科技计划项目(2010B020312005)

摘　　要：本试验采用超临界CO2萃取小球藻精油。并比较了精油和叶黄素的还原力和DPPH自由基清除率。利用GC-MS测定精油中不饱和脂肪酸,分析其抗氧化能力。结果表明,超临界CO2萃取所得的小球藻精油和提取纯化后所得的叶黄素晶体均具有良好的抗氧化性。纯化得到的小球藻叶黄素晶体总还原力大于小球藻精油,但DPPH自由基清除率小于小球藻精油。同时,小球藻精油含有种类丰富的不饱和脂肪酸,具有协同抗氧化作用。In this study,supercritical CO2 extraction was used to extract chlorella essential oil and lutein.The antioxidant activities（including the reducing power and DPPH radical scavenging ability） of the essential oil and lutein were compared.Besides,the content and the antioxidant activity of unsaturated fatty acids were determined by GC-MS.Results showed that essential oil extracted by supercritical CO2 and purified crystal lutein has excellent anti-oxidant activity.Purified crystal lutein exhibited higher total reducing power than oil,but lower the DPPH radical scavenging rate.It was also found that chlorella essential oils were rich in unsaturated fatty acids which have cooperative antioxidation.

关 键 词：小球藻 精油 抗氧化活性 超临界CO2萃取 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]