茵陈蒿汤和茵陈四逆汤抗二甲基亚硝胺诱导的大鼠肝纤维化的方证比较研究  

Recipe-syndrome Correlation Study of Yinchenhao Tang and Yinchen Sini Tang on Inhibiton of Liver Fibrosis Induced by Dimethylnitrosamine in Rats

作　　者：边艳琴[1,2] 刘平[1,2,3] 章李军[1,2] 宁冰冰[1,2] 陆雁[1,2] 陈高峰[1,2] 刘成[1,2] 刘佳[1,2] 孙明瑜[1,2,3] 

机构地区：[1]上海中医药大学附属曙光医院上海中医药大学肝病研究所,上海201203 [2]上海中医药大学肝肾疾病病证教育部重点实验室,上海201203 [3]上海高校中医内科学E-研究院,上海201203

出　　处：《辽宁中医药大学学报》2011年第8期68-71,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(90409020;30701070;81001486);上海市科学技术委员会启明星计划项目(08QA14062);上海中医药大学杏林学者项目;上海市重点学科建设项目(Y0302);上海市教育委员会E-研究院建设计划项目(E03008);上海高校创新团队建设项目;上海市中医临床重点实验室项目

摘　　要：目的:通过观察茵陈蒿汤和茵陈四逆汤对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化模型的效应,探讨该模型的证候病机。方法:大鼠随机分为正常组、模型组、茵陈蒿汤组和茵陈四逆汤组,采用腹腔注射DMN 4周诱导大鼠肝纤维化,于造模第3周开始,各给药组在继续造模的同时予以相应的药物干预,正常组和模型组给以等量生理盐水。4周末杀鼠取材,观察各组大鼠一般情况、肝组织病理学、羟脯氨酸(Hyp)含量及肝功能变化。结果:与正常组相比,模型组出现显著的肝损伤和肝纤维化,肝组织Hyp含量显著升高(P<0.01),肝功能显著异常(P<0.01)。与模型组比较,茵陈蒿汤可显著改善肝组织病理,降低肝组织Hyp含量及肝脏胶原增生程度(P<0.01),显著改善肝功能(P<0.05或P<0.01);而茵陈四逆汤不但不能改善任何指标,而且还加重了DMN诱导的大鼠肝脏的炎症和肝纤维化。结论:茵陈蒿汤对DMN诱导的大鼠肝纤维化模型的治疗效果显著优于茵陈四逆汤;DMN诱导的大鼠肝纤维化模型在其形成期的病机以"湿热内蕴"为主,且湿热并重,与茵陈蒿汤方证高度相关。Objective： To explore the recipe-syndrome correlation of Yinchenhao Tang（YCHT）and Yin-chen Si-ni Tang（YCSNT）on inhibition of liver fibrosis induced by dimethylnitrosamine in rats.Methods： Rats were randomized into four groups： control,DMN,YCHT,and YCSNT groups.Liver fibrosis model were induced by DMN intra-peritoneally injection for 4 weeks at the dosage of 10mg/kg body weight,once per day for 3 consecutive days in each week in rats.Each of groups was gastric infused respectively with distilled water（control or model group）,YCHT group and YCSNT in the continuous 2-week modeling period.At the end of the 4th week,rats were sacrificed and their blood sample and liver tissue were gotten for detection.Results： Liver fibrosis induced by DMN in rats was improved by administration of YCHT.YCHT treatment for 2 weeks,content of Hyp was decreased remarkably（P〈0.01）and liver function and hepatic histology were improved significantly（P〈0.05 or P〈0.01）;while those effects was not shown in the YCSNT groups.Conclusion： Efficacy of YCHT inhibiton of liver fibrosis induced by DMN in rats is far better than YCSNT.The pathogenesis of the liver fibrosis model is damp-heat syndrome.In addition,damp or heat pathogenic factor plays a very important role in this model.The syndrome is high correlation of YCHT.

关 键 词：方证研究 肝纤维化 茵陈蒿汤 茵陈四逆汤 

分 类 号：R-322[医药卫生]