LGT模型鼠血清及其癌组织提取液中蛋白质指纹比较研究  

The comparative analysis of the LGT protein fingerprints in model rat serum and cancerous tissue in model rat

作　　者：陈妍[1] 杜秀玉 裴毅[1] 

机构地区：[1]山西省肿瘤医院,030013 [2]山西省忻州市人民医院

出　　处：《中国医学创新》2011年第23期1-4,共4页Medical Innovation of China

摘　　要：目的 分析比较LGT模型鼠血清与其肿瘤组织中危重患者预警（LGT-Lost Goodwill Target）蛋白指纹的表达特征.方法 取4～5周龄雌性小鼠1只,接种S180肿瘤细胞悬液（02 ml/只）,建立小鼠S180肉瘤动物模型.肿瘤细胞株S180以腹水瘤形式在昆明（KM）小鼠体内传代,传代后第8天,无菌条件下抽取腹水并以生理盐水稀释,调整细胞密度 2×107/ml,制成肿瘤悬液,置于冰浴备用.将制备好的肿瘤细胞悬液以每只02 ml于昆明小鼠（10只）右腋下的皮下接种造模.建立小鼠S180肉瘤动物模型.随机分为造模组（A组,5只）及对照组（B组,5只）,肿瘤长至05 cm大小时,开始给予造模组（A组）腹腔注射顺铂52 mg/kg,连用4 d;同天给予空白对照组（B组）腹腔注射等计量生理盐水,连用4 d.于造模结束后第3天两组小鼠全部摘眼球取血,分离血清进SELDI 检测,解剖造模组小鼠,分别取瘤组织、瘤旁组织及正常组织各1 g,匀浆,抽取上清液行SELDI检查.并利用Biomarker Wizard 软件对各组血清及瘤组织、瘤旁组织及正常组织匀浆液的蛋白质组指纹图谱进行比较分析.结果 （1）A组血清LGT蛋白质组指纹阳性,B组血清LGT蛋白质指纹阴性.造模成功,A组可行进一步分析.（2）A组血清与癌组织液LGT相关蛋白质指纹图谱：有8个蛋白质差异有统计学意义（P〈005）.其m/z分别为1993、2009、2028、2217、2502、8696、9940、10145.（3）A组血清与癌旁组织液LGT相关蛋白质指纹图谱：有7个蛋白质差异有统计学意义（P〈005）.其m/z分别为1077、1211、1994、2009、2030、2081、11 817.（4）A组血清与正常组织液LGT相关蛋白质指纹图谱：有5个蛋白质差异有统计学意义（P〈005）.其m/z分别为1004、1994、2010、2030、2217.结论 本结论认为造成肿瘤恶化而导致死亡发生的因素极有可能不单独在肿瘤本身,而在于周围环境.各个部位所表达的蛋白质指纹也不一致,可以是因Objective To analyze and compare LGT model rat serum and tumor tissue LGT （ LGT - Lost Goodwill Target ） protein expression of fingerprint characteristics. Methods Take 4 - 5 weeks female mice 1 only, a mouse model was developed by inoculating S180 cells directly into KM mice. Tumor cell S180 with ascites tumor form in kunming （KM） mice nestin, the first eight days, after nestin under aseptic conditions and to extract ascites, adjust the diluted saline cell density 2 - 107/ml, make tumor levitation liquid, on the ice bath. Set aside. Will preparation good tumor cell suspension liquid per only 0. 2 ml in Kunming mice （10） only right armpit subcutaneous inoculation made moulds. All the mice were divided into 2 groups. When the tumor long to 0. 5 cm big hours , group A was injected cisplatin 5.2 mg/（ kg · d） for 4 days by intraper- itoneal injection. Group B was control groups for 4 days by with group A of intraperitoneal injection with days such as measuring saline 4 days. The mice were killed at the fourth day after chemotherapy end. Then we collected the eyeball blood samples, and SELDI was applicated to test it. Made of module mice anatomy, were taken tumor tissue, peritumoral organization and normal tissue 1 g each, use electric homogenate device homogenate, extraction superuatant fluid lines SELDI inspection. Respective proteomic fingerprint was analyzed by Biomarker Wizard software to discover the different proteomic fingerprints. Results （1）Group A serum LGT proteome fingerprint is positive, group B LGT serum protein fingerprint negative. （2） Group A serum protein with cancer organization fingerprint ： In the detection of protein fingerprint ： there are 9 protein difference was statistically significant （P 〈 0. 05 ）. Differences protein fingerprint m/z respectively： 1993,2009,1028,2217,2502, 2545,8696,99%, 10145. （3）Group A serum protein with peritumoral organization fingerprint： In the detection of protein fingerprint： there are 7 protein difference was statistic

关 键 词：模型鼠 SELDI技术 LGT 

分 类 号：R73-3[医药卫生—肿瘤]