海带愈伤组织的高效率诱导  

HIGHLY EFFICIENT CALLUS INDUCTION OF LAMINARIA JAPONICA

作　　者：王希华[1] 秦松[1] 曾呈奎[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》1999年第6期652-657,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金!39400076;国家攀登计划B资助

摘　　要：于1994年1月和11月在青岛太平角和荣成海带育苗场分别采集海带成熟孢子体和幼孢子体，采用培养基诱导方法，对4种培养基诱导海带愈伤组织的效果，成熟海带孢子体的不同部位在MS固体增富培养基中形成愈伤组织的能力进行了比较；研究了添加不同成分的MS固体增富培养基对海带愈伤组织形成的影响，并比较了4种除菌方法的效果。结果表明，PESI固体培养基和MS固体增富培养基是诱导海带愈伤组织的理想培养基，其诱导率分别为75．5％（24d）和67．3％（90d）。在MS固体增富培养基中培养120d后，生长部、假根和柄部的愈伤组织诱导率分别为80％、78％和67％。在MS固体增富培养基中同时添加6种成分，诱导率为12．5％；而当不添加任何成分时，诱导率为3．2％。1．5％用结合无菌水的处理方法，对海带组织块的除菌效果比较理想。To obtain a large amount of callus and to look for an effective method for bacteria elindnating, experiments were undertaken from tw, 1994 to July, 1996. The materials used were mature sporotwtes and young sporophtes of Laminaria japonica. the sporophytes were collected from Taipingiiao Bay, Qingdao in January, 1994, and young sporophytes were provided by Roncheng Feeding Farm in November, 1994.MS liquld medium; enriched MS solid medium [with mtritol (0.030g / m1), yeast extract (0.001g/nil), kinehn (0.107 6μg/Inl), NAA (1.86 μg/ml), VB2 and VB12, (0.5mg/ml each)]; ASP-C-Isolid medium and PESI solid medium were used. Solid medium was made from liquld medium by solidification with 1.5% agar Meristematic zone, shpe and rhizoid were cut off from the mature sporophtes. They were cllt into (2-3)mm×(2-3)mm pieces. Mannitol + yeast exhact VB2, + VB12'Kinetin + NAA and all above mentioned additives were added into MS soild mediuxn respechvely to compare their effects. Four tecboques for eliwhnating bacteria were tested: (1) 1.5%KI (W/V)+70%(V/D alcohol; (2) 1.5% KI+NaCIO (0.01g/m); (3) 1.5% KJ + autoclaved water; (4) antibiotics:Str., Str. + Lm., Amp. and Km. Young sporophytes were used in this test.Tissue pieces were cultured at 10.0±0.5℃, about 1 500 lx (10h light/ 14h clarknss) light All media were renewed every 15 days. Large amount of precipitates occrmd in MS liquld medium after 7 days and pH decreased during the culture, which affected the test No callus formed after five month of indution. No positive result was obtained using ASP-C-I solid medium, either. But in enriched MS solid medium (modified by all the additives mentioned above) and PESI solid medium, inductuve efficiency were 67.3% (90d) and 75.5% (24d), respechvely.By using different tissues in enriched MS solid medium, induhve efficiency was: meristematic zones > rhizoids > stipes (120d). By using sterilization method (3), none of the tissues was contaminated, bu aell tissues were coboinatod by using method (1)- Most tissues were contaminated by

关 键 词：海带 愈伤组织 组织培养 高效率诱导 

分 类 号：Q949.288.4[生物学—植物学]