核磁共振法测定硫酸依替米星含量  

Quantitative determination of etimicin sulfate by nuclear magnetic resonance

作　　者：于小波[1,2] 相秉仁[1,2] 王国华 宋喆[1,2] 沈文斌[1,2] 

机构地区：[1]中国药科大学分析测试中心,南京210009 [2]药物质量与安全预警教育部重点实验室,南京210009 [3]常州方圆制药有限公司,常州213022

出　　处：《中国抗生素杂志》2011年第8期610-612,634,共4页Chinese Journal of Antibiotics

摘　　要：通过核磁共振法对硫酸依替米星进行定性和定量分析。利用一维及二维核磁共振谱(DEPT、COSY、HSQC、HMBC),对1H-NMR谱和13C-NMR谱信号进行完整归属。在此基础上,采用氢核磁共振定量法,以对苯二酚为内标,测得硫酸依替米星中依替米星的含量为59.19%,RSD为0.24%,方法准确可靠,简便快速。Qualitative and quantitative analysis of etimicin sulfate were reported by nuclear magnetic resonance（NMR）. The ^lH-NMR and ^13C-NMR spectra of etimicin sulfate have been assigned by means of 1D and 2D spectroscopy including DEPT, COSY, HSQC and HMBC. On the basis of assignment, a novel approach was developed for the determination of purity of etimicin sulfate by proton-nuclear magnetic resonance （^1H-NMR） using p-hydroquinone as internal standard. The result showed that the purity of etimicin was 59.19%, and its relative standard deviations（RSD） was 0.24%, this method was relative accuracy, precision, and ease of application.

关 键 词：硫酸依替米星 核磁共振 含量 

分 类 号：R914.1[医药卫生—药物化学]