恶性疟原虫单表位单抗的建立及鉴定  

ESTABLISHMENT AND IDENTIFICATION OF THE MONOEPITOPIC MONOCLONAL ANTIBODY OF PLASMODIUM FALCIPARUM\+*

作　　者：瞿靖琦[1] 杨柏林[1] 包意芳[1] 杨玥涛[1] 许永湘[1] 许学年[1] 范培昌[2] 任一萍[2] 

机构地区：[1]中国预防医学科学院寄生虫病研究所,上海200025 [2]华东师范大学生物系,上海200062

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第6期338-341,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家"九五"科技攻关项目!(No.969060406)

摘　　要：目的： 研制恶性疟原虫单表位单抗。方法： 根据蛋白质结构理论， 选取能代表恶性疟原虫蛋白质抗原性的ＨＲＰ－ＩＩ肽段， 长度为６～９ 个氨基酸， 经人工合成， 通过毛细管电泳， 确定其纯度后， 对ＢＡＬＢ／ｃ小鼠进行免疫。结果： 应用脾脏埋植法和杂交瘤技术， 获得４ 株针对恶性疟原虫ＨＲＰ－ＩＩ单一表位的杂交瘤细胞。结论： 首次报告直接用合成ＨＲＰ－ＩＩ肽段制备针对该蛋白质的单表位单抗的方法获得成功。AIM: To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum. METHODS: According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine\|rich protein II (HRP\|II) of P.falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis. RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP\|II were obtaind by using spleen embedment method and hybridoma technology. CONCLUSION: It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.\;

关 键 词：合成肽 单表位单抗 恶性疟原虫 鉴定 

分 类 号：R382.31[医药卫生—医学寄生虫学]