三氯苯达唑对卫氏并殖吸虫体壁及卵黄细胞超微结构的影响  

EFFECT OF TRICLABENDAZOLE ON THE ULTRASTRUCTURE OF BODY WALL AND VITELLINE CELLS OF PARAGONIMUS WESTERMANI\+*

作　　者：许世锷[1] 陈彩云[1] 金立群[1] 陆秀君[1] 陈家俊[1] 

机构地区：[1]汕头大学医学院,汕头515031

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第6期342-345,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：广东省卫生厅及李嘉诚科研基金!资助项目(No.98026)

摘　　要：目的： 观察三氯苯达唑在体内外杀灭卫氏并殖吸虫后， 虫体体壁及卵黄细胞超微结构的变化。方法：将被药物在体内、外杀死的虫体及对照组正常虫体， 按常规方法制成电镜样品， 用扫描和透射电镜观察。结果： 在药物作用下， 体壁的外质膜及基质层裂解消失， 肌肉层有不同程度的坏死， 皮层细胞膜及卵黄细胞膜破坏消失，核膜部份破坏， 核内异染色质凝固、聚边、直至溶解。胞质内的高尔基体消失， 内质网扩张， 线粒体肿胀变性、直至溶解，但对糖原颗粒无明显影响。对体内虫体的破坏比体外严重。结论： 三氯苯达唑对卫氏并殖吸虫的体壁及卵黄细胞均有明显的破坏作用， 主要破坏细胞核、细胞的膜质结构以及微管系统， 并可使虫体皮层裂解消失。AIM: To observe the ultrastructural changes in the body wall and the vitelline cells of Paragonimus westermani in vitro and in vivo before and after triclabendazole treatment. METHODS: The worms were obtained from in vitro and in vivo tests. All of the samples were processed by conventional techniques, and observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). RESULTS: The external plasma membrane and matrix were cracked or disappeared after the treatment. The necrosis of the muscular layer differed. The cell membranes of cortex and vitelline cells were damaged. Nuclear membrane was damaged partially, heterochromatin solidified and condensed to brim and dissolved. The Golgi complex disappeared, endoplasmic reticulum expanded, mitochondria denatured and dissolved. The damage was more serious in vivo than in vitro. CONCLUSION: Triclabendazole is remarkablely effective against Paragonimus westermani by damaging the body wall and vitelline cells, mainly affecting the nuclei, membrane structures and microtubular system.\;

关 键 词：三氯苯达唑 卫氏并殖吸虫 体壁 卵黄细胞 

分 类 号：R383.23[医药卫生—医学寄生虫学] R532.220.5[医药卫生—基础医学]