铁调节蛋白质-2对肺癌铁代谢调节作用的实验研究  

Regulating action of iron regulatory protein-2 in iron metabolism of lung cancer

作　　者：程哲[1] 刘玉峰[2] 宋永娜[1] 代灵灵[1] 康燕[1] 夏杰[1] 司纪明[1] 陈春艳[1] 

机构地区：[1]郑州大学第一附属医院呼吸二科河南省高等学校临床医学重点学科开放实验室,450052 [2]郑州大学第一附属医院儿科,450052

出　　处：《中华医学杂志》2011年第28期1992-1995,共4页National Medical Journal of China

基　　金：河南省卫生科技创新型人才工程（豫卫科2010-52）;河南省医学科技攻关项目（2009A320018）

摘　　要：目的 探讨铁调节蛋白质-2（IRP2）在肺癌铁代谢中的调节作用.方法 培养肺腺癌A549细胞随机分成脂质体组（含脂质体20 mg/L）、对照寡核苷酸组和反义寡核苷酸组3组,每组设10个平行样.通过脂质体介导转染,以脂质体组和对照寡核苷酸组为对照.利用逆转录-PCR、Western印迹检测转铁蛋白、转铁蛋白受体、铁蛋白等铁代谢相关基因mRNA及蛋白的表达.结果 转染48 h后,转铁蛋白mRNA在3组之间表达差异无统计学意义（F=2.18,P=0.078）;反义寡核苷酸组转铁蛋白受体mRNA表达显著低于脂质体组和对照寡核苷酸组（均P〈0.05）;反义寡核苷酸组铁蛋白mRNA（0.56±0.06）表达高于脂质体组（0.36±0.05）和对照寡核苷酸组（0.39±0.03）（均P〈0.05）.转染48 h后,反义寡核苷酸组IRP2蛋白表达明显低于脂质体组和对照寡核苷酸组（P〈0.05）;转铁蛋白在3组之间表达差异无统计学意义（F=2.668,P=0.088）;反义寡核苷酸组转铁蛋白受体蛋白表达低于脂质体组和对照寡核苷酸组（P〈0.05）;反义寡核苷酸组铁蛋白表达高于脂质体组和对照寡核苷酸组（P〈0.05）.结论 IRP2可能通过蛋白量的改变来影响肺腺癌A549细胞转铁蛋白受体和铁蛋白的表达,调节铁代谢.Objective To discuss the regulating mechanism of iron regulatory protein-2 （IRP2） in the iron metabolism of lung cancer. Methods The cultured A549 cells were divided into 3 groups： liposome group （including liposomes 20 mg/L）, random oligonucleotide group （SCODN group） and antisense oligonucleotide group （ASODN group）. And the liposome-mediated transfection was employed with the liposome and SCODN groups as controls. Reverse transcription-polymerase chain reaction （RT-PCR） and Western blot were used to examine the mRNA and protein expressions of iron metabolism-related transferring （Tf）, transferrin receptor （TfR） and ferritin （Fn） genes, etc. Results After a 48-hour transfection, the mRNA expression of Tf had no statistically significant difference among three groups （F=2.18, P=0.078）; the mRNA expression of TfR in the ASODN group was significantly lower than that in the liposome and SCODN groups （P〈0.05）. The expression of Fn mRNA in the ASODN group （0.56±0.06） was higher than that in the liposome （0.36±0.05） and SCODN groups （0.39±0.03） （P〈0.05）. After a 48-hour transfection, the IRP2 protein expression of the ASODN group was significantly lower than those of the liposome and SCODN groups （P〈0.05）. The Tf protein expression was not statistically different in three groups （F=2.67, P=0.088）. The TfR protein expression of the ASODN group was lower than those of the liposome and SCODN groups （P〈0.05）. And the Fn protein expression of the ASODN group was higher than those of the liposome and SCODN groups （P〈0.05）. Conclusion IRP2 may affect the expressions of TfR and Fn in lung adenocarcinoma A549 cells by changing the amount of protein and regulating the iron metabolism.

关 键 词：腺癌 细支气管肺泡 铁调节蛋白质-2 寡核糖核苷酸类 反义 转铁蛋白 铁蛋白质类 

分 类 号：R734.2[医药卫生—肿瘤]