胃肠道间质瘤64例免疫标记物应用分析  

Application of immunohistochemistry to the diagnosis of gastrointestinal stromal tumors of 64 cases

作　　者：夏庆欣[1,2] 赵驰[3] 庄競[1,4] 乔思杰[1,2] 

机构地区：[1]河南省肿瘤医院 [2]郑州大学附属肿瘤医院病理科,郑州450003 [3]郑州大学第四附属医院病理科,郑州450044 [4]郑州大学附属肿瘤医院普外科,郑州450003

出　　处：《临床与实验病理学杂志》2011年第7期698-701,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨胃肠道间质瘤(gastrointestinal stromal tumor,GIST)的免疫组化标记物的表达特征,为其诊断及鉴别诊断提供依据。方法收集85例确诊的胃肠道及腹、盆腔间叶源性肿瘤标本,其中GIST64例。免疫组化法检测CD117、CD34、DOG1、NSE、S-100、SMA和vimentin的表达。结果 GIST组中CD117、CD34、DOG1、NSE阳性率分别为89.1%、76.6%、96.9%及87.5%;非GIST组中的阳性率分别为4.8%、33.3%、28.6%和50.0%。CD117阴性的GIST组中CD34、NSE、DOG1阳性率分别为28.6%、71.4%和85.7%。结论 GIST中DOG1表达的敏感性高于CD117,在CD117阴性的病例中DOG1优于其他标记物。DOG1与CD117及NSE等联合应用于免疫组化检测足以明确绝大多数GIST的诊断。Purpose To investigate the ability of immunohistochemical markers for the diagnosis and differential diagnosis of gastrointestinal stromal tumors（GISTs）.Methods 85 cases of diagnosed gastrointestinal,pelvic or abdominal mesenchymal tumors were collected,including 64 gastrointestinal stromal tumors,and subjected to immunohistochemistry with antibodies to CD117,CD34,DOG1,NSE,S-100,SMA and vimentin.Results The staining results showed that in GISTs,the positive rates of CD117,CD34,DOG1 and NSE were 89.1%,76.6%,96.9% and 87.5% respectively.In non-GISTs,the positive rates of CD117,CD34,DOG1 and NSE were 4.8%,33.3%,28.6% and 50.0% respectively.In CD117-negative GISTs,the positive rates of CD34,NSE and DOG1 were 28.6%,71.4% and 85.7%.Conclusions The expression of DOG1 in gastrointestinal stromal tumors is more sensitive than CD117 and other immunomarkers especially in CD117-negative GISTs.In most cases of suspected GISTs a combination of CD117,DOG1 and NSE immunostaining should be sufficient to confirm the diagnosis.

关 键 词：胃肠道肿瘤 间质瘤 DOG1 CD117 免疫组化 

分 类 号：R735[医药卫生—肿瘤]