塞来昔布对鼻咽癌CNE-2Z细胞株CD44v6表达水平的影响  

Influence of celecoxib on the expression of CD44v6 in human nasopharyngeal carcinoma CNE-2Z cell line

作　　者：罗伟仁[1] 黎思怡[2] 李丽霞[3] 邓惠华[1] 姜汉国[1] 陈小毅[1] 

机构地区：[1]广东医学院病理学教研室,东莞523808 [2]广东医学院第二临床学院,东莞523808 [3]广东医学院附属医院肿瘤中心,东莞523808

出　　处：《临床与实验病理学杂志》2011年第7期731-734,738,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：广东省自然科学基金项目(031963)

摘　　要：目的研究塞来昔布(COX-2选择性抑制剂)对鼻咽癌CNE-2Z细胞侵袭能力的影响及可能机制。方法噻唑蓝(MTT)法检测塞来昔布对鼻咽癌CNE-2Z细胞增殖活性的影响。不同浓度塞来昔布作用鼻咽癌CNE-2Z细胞24 h后,通过重组人工基膜实验检测细胞侵袭力的改变;RT-PCR检测鼻咽癌细胞CD44v6 mRNA的表达情况;用Western blot和免疫细胞化学检测鼻咽癌细胞CD44v6蛋白表达变化。结果 MTT法结果显示,塞来昔布对鼻咽癌CNE-2Z细胞增殖有明显抑制作用,呈剂量依赖性关系(P<0.01)。塞来昔布作用细胞24 h后,肿瘤细胞的侵袭力明显下降(P<0.05)。RT-PCR结果显示,塞来昔布可以明显抑制CD44v6 mRNA的表达水平(P<0.01)。Western blot和免疫组化结果显示,随着药物浓度的增加,CD44v6蛋白表达水平逐渐减少(P<0.01)。结论塞来昔布可能通过下调CD44v6表达而降低鼻咽癌CNE-2Z细胞侵袭能力。Purpose To evaluate the effect and mechanism of celecoxib（a selective cyclooxygenase-2 inhibitor） on the invasive ability of human nasopharyngeal carcinoma（NPC） line CNE-2Z.Methods MTT assay was used to detect the effect of celecoxib on NPC cell proliferation.After treated with different concentrations of celecoxib for 24 hours,the reconstituted basement membrane invasion assay was used for evaluating the changes of NPC cell invasion capacity.CD44v6 mRNA expression was detected by reverse transcription-polymerase chain reaction（RT-PCR）.Western blot and immunocytochemistry were used to detect CD44v6 protein expression.Results MTT results indicated NPC cell proliferation was significantly suppressed by celecoxib in dose-dependent manner（P0.01）.After treated with celecoxib for 24 hours,NPC cell invasion capacity was significantly decreased compared with control group（P0.05）.RT-PCR results indicated that CD44v6 mRNA expression was significantly suppressed by celecoxib（P0.01）.Western blot and immunocytochemistry results suggested that CD44v6 protein expressions were significantly down-regulated with celecoxib concentrations increased（P0.01）.Conclusions Celecoxib inhibits invasion ability of NPC cell due to the down-regulation of CD44v6 expression.

关 键 词：鼻咽肿瘤 环氧化酶-2 塞来昔布 侵袭 

分 类 号：R739.63[医药卫生—肿瘤] R392.31[医药卫生—临床医学]