听神经瘤组织中轴突神经丝及髓鞘碱性蛋白表达的检测及其意义  

Detection of expressions of neurofilament and myelin basic protein in acoustic tumor tissues and its significances

作　　者：王琰[1] 姜海洋[1] 于何[1] 关超[1] 姜学钧[1] 

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉科,辽宁沈阳110001

出　　处：《吉林大学学报（医学版）》2011年第4期688-690,774,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金科学部主任基金资助课题(30540080)

摘　　要：目的:检测听神经细胞瘤组织中轴突神经丝及髓鞘碱性蛋白的表达情况,探讨听神经瘤组织中组织细胞的生长特点。方法:选取本院听神经瘤患者的手术组织15例(听神经瘤组)及正常神经5例(正常对照组),应用免疫荧光标记及免疫印迹方法,检测神经组织中的轴突神经丝200蛋白及髓鞘碱性蛋白的表达情况。结果:听神经瘤组织中,轴索的标志性抗体——神经丝200的表达为阴性,并且启动髓鞘化的前哨信号及髓鞘致密化的重要组成蛋白——髓鞘碱性蛋白在听神经瘤组织中也未检测到。而在正常对照组,二者的表达均为阳性。结论:在听神经瘤组织中缺失致密的髓鞘及轴索,提示听神经瘤细胞可能是处于前髓鞘化期的施万细胞,并且该类细胞失去了完成髓鞘化进程和重新形成致密髓鞘的能力。Objective To detect the expressions of neurofilament and myelin basic protein in acoustic tumor tissues and explore the growth features of cells in acoustic tumor tissues.Methods Immunofluorescence labeling and immunoblotting analysis were performed to detect the expressions of neurofilament and myelin basic protein in acoustic tumor tissues in 15 patients with acoustic tumor（acoustic tumor group） and 5 cases of normal peripheral nerves（control group）.Results The expression of myelin basic protein indicating the beginning of myelination in acoustic tumor tissues was negative.The expression of axon marker neurofilament was also negative.But the expressions of neurofilament and myelin basic protein in control group were positive.Conclusion The axon or myelin are compact abscent in acoustic tumor tissues,it indicates that the cells of acoustic tumor is in a premyelinating state,and lose the ability to form compact mylin or remyelination.

关 键 词：髓鞘碱性蛋白 听神经瘤 神经丝蛋白 

分 类 号：R730.264[医药卫生—肿瘤]