大鼠实验性牙移动过程中三叉神经节内甘丙肽表达的研究  

Expression of Galanin in Trigeminal Ganglion of Rat during the Experimental Tooth Movement

作　　者：贾亚梅[1] 孙秀梅[1] 孙新华[1] 郑义[2] 

机构地区：[1]吉林大学口腔医院正畸科,吉林长春130021 [2]吉林大学口腔医院牙周病科

出　　处：《口腔医学研究》2011年第7期571-574,共4页Journal of Oral Science Research

摘　　要：目的:研究实验性牙移动过程中大鼠三叉神经节内甘丙肽表达的变化,探讨其与正畸疼痛缓解之间是否具有内在的联系。方法:选择雄性Wistar大鼠35只,建立正畸牙移动动物模型,将大鼠随机分为实验组与空白对照组。实验组左侧为加力侧,右侧为未加力侧,左右自身对照,分别于加力6h、12h、1、3、5、7d后处死动物,取大鼠三叉神经节,标本制备,进行免疫组织化学染色与图像分析。采用SPSS16.0统计学软件进行统计学分析。结果:与空白对照组相比,实验组加力1d甘丙肽阳性细胞数增多,表达增强(P<0.05);加力3d阳性细胞数增多明显(P<0.01);加力5d甘丙肽免疫阳性细胞数达到高峰,神经节细胞中甘丙肽免疫阳性染色呈强阳性表达(P<0.01)。实验组未加力侧各时间点与空白对照组之间甘丙肽表达无统计学差异。结论:GAL作为内源性镇痛物质与正畸牙移动所致牙体与牙周疼痛的缓解具有一定的内在联系。Objective： To observe the changes oi gatallin and to study the relation between the galanin intrinsically and the relief of pain. Methods： 35 male rats were selected and randomly divided into the experiment group （30rats） and the control group （Srats）, and the orthodontic tooth movement model was made. The left first molar of the rats in experimental group was pulled toward mesiocclusion by a kind of spring, and the right side was not. The rats were sacrificed after 6 hours, 12 hours, 1 day, 3 days, 5 days and 7 days, respectively, and the trigeminal ganglion were extracted from the head. Then, the immunohisto- chemical stain for galanin in the trigeminal was carried out. SPSS16.0 statistical software was used. Results： Com- pared with the control group, the number of GAL--immunoreactive ganglion cells in experimental trigemina[ in- creased after 1day treated by 50g force. After 3 days, GAL--immunoreactive ganglion cells in experimental trigemi- nal increased conspicuously. The peak appeared after 5 days. Only a few of GAL--immunoreactive ganglion cells appeared in the control side and there was no significantly change. Moreover, there was ao significant difference be- tween the control group and the right side of the experimental rats. Conclusion： As an alleViation, GAL has a certain internal relation with dental pain relieving caused by the tooth movement.

关 键 词：甘丙肽牙移动疼痛 

分 类 号：R783.5[医药卫生—口腔医学]