涎腺粘液表皮样癌中Caveolin-1、PCNA的表达及意义  

Expression and Significance of Caveolin-1 and PCNA in Mucoepidermoid Carcinoma of Salivary Glands

作　　者：史璐[1] 陈新明[2] 陈智[3] 熊世春[2] 李原[2] 

机构地区：[1]郑州大学口腔医学院牙体牙髓科,河南郑州450000 [2]武汉大学口腔医学院病理科 [3]武汉大学口腔医学院牙体牙髓科

出　　处：《口腔医学研究》2011年第7期596-599,共4页Journal of Oral Science Research

摘　　要：目的:研究Caveolin-1、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在涎腺粘液表皮样癌中的表达,分析二者的关系及其与肿瘤生物学行为间的关系。方法:采用SP免疫组化技术检测75例涎腺粘液表皮样癌标本及20例正常涎腺组织中Caveolin-1、PCNA的表达,并行统计学分析。结果:Caveolin-1在20例正常涎腺组织中均呈阳性表达。Caveolin-1表达下调与病程短、病理级别及临床分期的增高有关,短病程组和长病程组间(42.1%vs 67.6%)、低分化和高分化组间(38.5%vs 63.3%)、Ⅲ+Ⅳ期和Ⅰ+Ⅱ期组间(33.3%vs 64.7%)比较差异有统计学意义(P<0.05)。PCNA随着病理级别和临床分期的增高阳性表达率升高,低分化和高分化组间(33.38±11.98vs 20.12±10.13)、Ⅲ+Ⅳ期和Ⅰ+Ⅱ期组间(29.45±12.89vs 22.49±11.73)比较差异有统计学意义(P<0.05)。Caveolin-1与PCNA的表达呈负相关(rs=-0.28,P=0.017)。Caveolin-1表达降低及PCNA表达增高与肿瘤复发显著相关(P<0.05)。结论:Caveolin-1的表达对粘液表皮样癌细胞增殖起抑制作用,Caveolin-1是影响粘液表皮样癌发生发展的重要因素之一。Objective： To investigate the expression and significance of caveolin- and proliferating cell nuclear antigen （PCNA） in mucoepidermoid carcinoma （MEC） of salivary glands. Methods： The expression of caveolin-1 and PCNA in MEC tissues （75 cases） and normal salivary glands tissues （20 cases, control group） were detected by immunohistochemical SP assay. Results.Caveolin-1 expression was presented in all normal salivary gland sam- pies. Reduced caveolin-1 expression was associated with shorter duration of tumor（shorter group vs longer group = 42.1% vs 67.6 %）, higher grade malignance （high-grade vs lowgrade = 38. 5 % vs 63.3 %） and advanced clinical stage（Ⅲ＋vs I ＋Ⅱ=29. 454±12.89 vs 22.49±11.73） （P〈0.05）. There were significant differences in the expression of PCNA between high--grade MEC and low--grade MEC （33. 384-11.98 vs 20. 124-10.13） Ⅲ＋ IVstages and I 4Ⅱtages （29.45＋12.89 vs 22. 494-11.73） （P〈0.05）. The expression of caveolin-- 1 was nega- tively related with the expression of PCNA （rs= -.28, P = 0. 017）. Reduced caveolin-1 expression and increased PCNA expression were positively correlated with unfavorable outcome. Conclusion.. The currant data indicated a re- duction in caveolin- expression in MEC of salivary glands, which inversely correlated with patients＂ outcome and tumor cell proliferation, suggesting its role as a tumor supressor in MEC of salivary glands.

关 键 词：涎腺粘液表皮样癌 CAVEOLIN-1 增殖细胞核抗原 

分 类 号：R739.8[医药卫生—肿瘤]