HIV-1包膜蛋白V2区突变对V3区特异的中和抗体的影响  

Impact of mutation at V2 of gpl20 on V3-specific neutralizing antibodies

作　　者：刘沐桑[1] 松下修三 柴田润二[2] 刘维达[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所,南京210042 [2]日本熊本大学艾滋病中心

出　　处：《中华微生物学和免疫学杂志》2011年第7期613-617,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究HIV－1包膜蛋白、吃区突变对V3区特异中和抗体与HIV－1中和作用的影响。方法使用假病毒中和试验测定L175P突变对于V3区中和抗体对HIV-1中和能力的影响，ELISA方法测定上述中和抗体对gp120蛋白单体的结合力。结果V2区突变L175P可以改变多个V3区特异性中和抗体对病毒的中和能力，但这些中和抗体对gp120蛋白单体的结合力不因V2区突变而改变。结论V2区的L175P突变改变了gp120蛋白天然三聚体的结构，促进了V3区抗体的中和能力。Objective To study the impact of V2 mutations on neutralizing ability of HIV-l-specific neutralizing antibodies. Methods We tested the influence of L175P mutation to the neutralizing ability of V3-specific antibodies by pseudotype virus and the binding affinity of those V3-spesific antibodies to gpl20 monomer by ELISA. Results We found L175P mutation changed the neutralizing ability of V3-specific antibodies. However, L175P mutation showed no effects on the binding affinity of these antibodies to gpl20 monomer. Conclusion Our results revealed the L175P mutation at V2 loop changed the natural trimmer structure of gpl20 and enhanced the neutralizing ability of V3-speeific antibodies.

关 键 词：HIV V2区 V3区 突变 中和抗体 

分 类 号：R392[医药卫生—免疫学]