鼠IL-28重组腺病毒体外抗肺癌活性研究  

The vitro anticancer activity analysis of mouse IL-28 recombinant adenovirus vector

作　　者：严玉兰[1] 张金[2] 刘洋[1] 袁利学[2] 朱涛峰[2] 郑金旭[2] 步雪峰[1] 

机构地区：[1]江苏省镇江市江苏大学附属人民医院呼吸内科,212002 [2]江苏大学临床医学院

出　　处：《中华微生物学和免疫学杂志》2011年第7期638-642,共5页Chinese Journal of Microbiology and Immunology

基　　金：卫生部基金项目资助（wkj2006-02-206）

摘　　要：目的将已成功构建的mlL-28A重组腺病毒载体转染至肺腺癌细胞LA795，并对其抗肺癌细胞生物学活性进行研究。方法将Ad—pshuttle—cmv—mlL-28A转染至LA795细胞，用PCR、免疫细胞荧光、Tunel、AnnexinV及MTT法等进行检测。结果LA795细胞转染Ad—mlL-28A后，mlL-28A的mRNA基因表达明显增加，且细胞内明显表达IL-28蛋白，LA795细胞凋亡增多，细胞生长明显抑制。结论成功构建的mlL-28A重组腺病毒载体转染至肺腺癌细胞LA795后表达IL-28，且可能通过促进细胞凋亡而抑制其-定程度的生长。Objective To transfect the recombinant mIL-28A adenovirus vector into lung adenocarcinoma cell line LA795 and research its anticancer activity. Methods Transfected the constructed mouse IL-28 （raiL-28） recombinant adenovirus vector into LA795 cell line, detected with PCR, immunocytal fluorescence, Tunel, Annexin V and MTI＇. Results Transfected with rAd-mlL-28A into the LA795 cells, mlL-28A gene expression products mRNA increased obviously, IL-28 expression was detected in cells obviously, apoptosis cell number increased, and the growth of LA795 cells transfeeted with rAd-mlL-28A were inhibited obviously. Conclusion The recombinant mlL-28A adenovirus vector we have constructed, which expresses IL-28 when transfected to lung adenocareinoma cell line LA795, inhibits growth of carcinoma cell to some extent, and may work by promoting the apoptosis of cancer cells.

关 键 词：mIL-28 腺病毒 肺癌 生长 凋亡 

分 类 号：R734[医药卫生—肿瘤]