胰腺癌组织S100A4和MMP-9表达及其预后意义的探讨  

Correlation of both S100A4 and MMP-9 expressions with the prognosis of pancreatic cancer patients

作　　者：贾富鑫[1] 刘江伟[1] 张东[1] 李鹏[1] 李建英[2] 卢开柏[2] 

机构地区：[1]兰州军区乌鲁木齐总医院肝胆外科,新疆乌鲁木齐830000 [2]兰州军区乌鲁木齐总医院病理科,新疆乌鲁木齐830000

出　　处：《中华肿瘤防治杂志》2011年第14期1105-1109,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：中国博士后科学基金第48批面上资助项目(20100481517);兰州军区医药卫生科研基金(LXH-2005019)

摘　　要：目的:探讨钙离子结合蛋白A4(S100A4)和基质金属蛋白酶9(MMP-9)基因在胰腺癌组织中的表达和临床病理因素及预后的关系。方法:采用免疫组化二步法检测63例手术切除的原发性胰腺癌组织中S100A4和MMP-9的表达情况,比较S100A4和MMP-9蛋白表达与临床病理因素的关系及对预后的影响。结果:63例胰腺癌标本中S100A4和MMP-9阳性率分别为74.6%(47/63)和68.3%(43/63)。S100A4和MMP-9蛋白过表达存在显著相关性,P=0.000。S100A4和MMP-9表达均与TNM分期、淋巴结转移和远处转移显著相关,同时MMP-9的表达也与肿瘤的大小及分化程度存在显著相关。S100A4阴性/MMP-9阴性患者(13例)中位生存时间(23个月)明显大于S100A4阳性/MMP-9阳性患者(36例)中位生存时间(9个月),χ2=15.353,P=0.000。分化程度(P=0.000)、淋巴结转移(P=0.003)和S100A4过表达(P=0.001)及MMP-9过表达(P=0.001)为影响预后的独立因素。结论:S100A4和MMP-9的高表达与临床病理参数密切相关两者在胰腺癌的生长浸润过程中起重要作用,可以作为判断预后的指标之一。OBJECTIVE： To study the expression of calcium-binding protein S100A4 and matrix metalloproteinases-9（MMP-9） in pancreatic cancer and their correlation with clinicopathological parameters and prognosis of pancreatic cancer patients.METHODS： The expressions of S100A4 and MMP-9 were examined in 63 surgical specimens of primary pancreatic carcinoma with immunohistochemistry methods.Correlation of these expressions with clinicopathological parameters and prognosis of pancreatic cancer was analyzed.RESULTS： Forty-seven out of 63（74.6%） specimens of primary pancreatic cancer were positive for S100A4 and Forty-three of 63（68.3%）pancreatic cancer specimens showed positive expressions of MMP-9 according to immunohistochemistry detection.The positive expressions of S100A4 and MMP-9 were significantly correlated with pancreatic cancer（P=0.000）.The expressions of S100A4 and MMP-9 were significantly correlated with TNM stages,lymph node metastasis and distant metastasis.The expressions of MMP-9 was also significantly correlated with the tumor size and differentiation.The median survival（23 months） of 13 patients with S100A4（-）/MMP-9（-） was significantly longer than the median survival（9 months） of 36 patients with S100A4（＋）/MMP-9（＋）,χ2=15.353,P=0.000.The differentiation（P=0.000）,lymph node metastasis（P=0.003）,S100A4（＋） expression（P=0.001） and MMP-9（＋） expression（P=0.001） were independent prognostic factors for pancreatic cancer.CONCLUSIONS：The overexpressions of S100A4 and MMP-9 proteins were related with clinicopathological parameters of pancreatic cancer patients and play important roles in proliferation and progression in pancreatic cancer.The combined examination of these two molecules is useful in evaluating the prognosis of pancreatic cancer patients.

关 键 词：胰腺肿瘤 免疫组织化学 S100A4 MMP-9 预后 

分 类 号：R735.9[医药卫生—肿瘤]