乳腺癌中组蛋白乙酰化与p21^WAF1的表达研究  

The expression research of histone acetylation and p21^WAF1in breast cancer

作　　者：赵洁[1] 罗学锋[1] 刘晓颖[1] 伍玲[1] 

机构地区：[1]湖南省马王堆医院病理科,长沙410016

出　　处：《中国医师杂志》2011年第7期876-879,共4页Journal of Chinese Physician

基　　金：湖南省科技厅基金（2008FJ322）

摘　　要：目的探讨乳腺癌中乙酰化的组蛋白H3、H4及p21^WAF1的表达变化及其意义。方法采用HE染色鉴定乳腺癌的病理形态变化；应用免疫组织化学法检测p21^WAF1在80例乳腺癌组织与80例正常乳腺组织中的表达；免疫印迹法检测乙酰化的组蛋白H3、H4及p21^WAF1蛋自在80例乳腺癌组织及80例正常乳腺组织中的表达。结果·HE染色可见，与正常的乳腺组织相比，乳腺癌组织结构及细胞形态有明显的异型性。免疫组化结果显示：p21^WAF1。在80例乳腺癌组织中有49例阳性表达（61．25％）；而80例正常乳腺组织中只有3例弱阳性表达（3．75％），两种组织中的表达差异有统计学意义（P〈0．05）。免疫印迹法结果表明，p21^WAF1蛋白在乳腺癌中的表达高于正常组织，乳腺癌组为0．78±0．095、正常乳腺组为0．65±0．055；乙酰化的组蛋白H3、H4在正常乳腺组织中的表达比乳腺癌组织高，其中乙酰化的组蛋白H3正常乳腺组为2．35±0．340、乳腺癌组为1．07±0．067，乙酰化的组蛋白H4正常乳腺组为3．44±0．202、乳腺癌组为1．11±0．086，两者表达差异均有统计学意义（P〈0．01）。结论组蛋白乙酰化与p21^WAF1蛋白的表达变化与乳腺癌的发生发展有关。Objective To research the expression change and the sense of histone acetylation and p21^WAF1 protein in breast cancer. Methods Pathological morphologic changes of breast cancer were identified by H. E. staining. Immunohistochemieal study for p21^WAF1 protein expression was performed on 80 breast cancers and 80 normal breast tissues. The expressions of acetylated histone H3, H4 and p21wan protein in 80 breast cancers and 80 normal breast tissues were analyzed by Western blot. Results H.E. staining discovered that the tissue structure and cell morphous of breast cancer had visible atypia, compared with normal breast tissues. The immunohistochemical results displayed that the positive expression of p21^WAF1 in 80 breast cancers and 80 normal breast tissues was 49 cases（ 61.25 % ） or 3 cases （3.75%）, respectively. There was significantly difference in the expression level between the 80 breast cancers and 80 normal breast tissues （ P 〈 0. 05）. The expression levels of p21^WAF1 protein in breast cancer tissues were higher than that in normal breast tissues, 0. 78 ± 0. 095 or 0. 65 ±0. 055, respectively. The expression levels of aeetylated histone H3, H4 protein in normal breast tissues were higher than those in breast cancer tissues. H3 was 2. 35 ± 0. 340 or 1.07 ± 0. 067, respectively, H4 was 3.44 ± 0. 202 or 1.11±0. 086, respectively. There was significantly difference in the expression levels between the 80 breast cancers and 80 normal breast tissues （ P 〈 0.01 ）. Conclusions The occurrence and development of breast cancers may be related to the expression change of histone acetylation and p21 WAn protein.

关 键 词：乳腺肿瘤/代谢/病理学 组蛋白类/生物合成 原癌基因蛋白质p21(ras)/生物合成 乙酰化作用 

分 类 号：R737.9[医药卫生—肿瘤]