miR-149促进鼻咽癌细胞侵袭和上皮-间质转变(英文)  

miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells

作　　者：罗朝辉[1] 张李洋[1] 李征[1] 江琛[1] 代亚飞[1] 刘晓萍[1] 郑瑛[1] 俞海波[1] 向娟娟[1] 李桂源[1] 

机构地区：[1]中南大学肿瘤研究所,长沙410078

出　　处：《中南大学学报（医学版）》2011年第7期604-609,共6页Journal of Central South University ：Medical Science

基　　金：supported by the National Natural Science Foundation of China(81000882,81000972)

摘　　要：目的:探讨miR-149在鼻咽癌中的功能和机制。方法:Real-time PCR和2-△△Ct计算方法验证miR-149在鼻咽癌细胞系中的表达,分别应用MTT实验、划痕实验和transwell迁移实验分析miR-149对鼻咽癌细胞的增殖、迁移和侵袭功能的影响。Western印迹检测E-cadherin的变化。结果:相对于正常鼻咽上皮细胞NP69,miR-149在5-8F和6-10B鼻咽癌细胞系中表达增高。MTT实验、划痕实验和transwell实验显示miR-149能够促进鼻咽癌细胞的增殖、迁移和侵袭。Western印迹证实miR-149能够降低E-cadherin的表达;反之,抑制miR-149能抑制鼻咽癌细胞的增殖、迁移和侵袭转移能力。结论:miR-149通过调节细胞上皮-间质转变在鼻咽癌的侵袭转移中发挥重要作用。Objective To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma（NPC）.Methods The expression of miR-149 was examined by real-time PCR and calculated by 2-△△Ct method.The cell proliferation was analyzed by MTT assay.The cell migration and invasion were shown by the wound healing assay and transwell migration assay,and the expression of E-cadherin was detected by Western blot.Results The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69.MTT assay showed that miR-149 promoted the proliferation of NPC cell lines.The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines.Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade.miR-149 downregulated the expression of E-cadherin,whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin.Conclusion miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition（EMT）.

关 键 词：miR-149 鼻咽癌 迁移 侵袭转移 上皮-间质转变 

分 类 号：R739.63[医药卫生—肿瘤]