神经生长因子及其受体在下颌骨骨折愈合中的表达及意义  

Expression of nerve growth factor and receptors during mandible fracture healing

作　　者：于立明[1] 张伟[2] 陈坤[1] 吴亚东[2] 

机构地区：[1]广东医学院附属医院口腔颌面外科,湛江524000 [2]吉林大学口腔医学院

出　　处：《实用口腔医学杂志》2011年第4期460-464,共5页Journal of Practical Stomatology

摘　　要：目的:探讨神经生长因子(nerve growth factor,NGF)及其受体(p75和TrkA)在骨折愈合过程的表达及其对骨折愈合的影响。方法:将32只白兔随机分为8组(每组4只),4只无骨折作为健康对照组2,8只制作右下颌骨骨折模型,立即行钛板固定。术后实验组动物分别在1、3、5、7、14、21、28 d各取4只处死。切取下颌骨脱钙后制作骨组织切片,行HE染色、NGF及受体的免疫组化染色。结果:NGF在骨折愈合过程中表达阳性3,～5 d达到高峰,在未骨折组仅有轻度阳性表达。NGFR-TrkA在骨折愈合过程中表达强阳性7,d达到高峰,到21 d时软骨细胞强阳性。NGFR-p75在骨折愈合过程中表达阳性,5～7 d达到高峰,到21 d时仍为阳性表达。结论:在骨折愈合过程中NGF、p75和TrkA共同调节骨细胞活性,对骨的修复重建起到重要作用。Objective： To study the expression of nerve growth factor（NGF） and receptors（p75 and TrkA） during bone fracture healing.Methods： 32 rabbits were devided into 8 groups randomly.4 of them without fracture were used as the controls.Right mandible fracture model was created in 28 rabbits,then the fractures were fixed with titanium plates immediately.Animals of experimental group were executed at 1,3,5,7,14,21,28 d after fracture respectively.Right mandibles and soft tissue around them were prepared for HE staining and immunohistochemical assay for NGF and receptor expression.Results： NGF expression was stronger in fracture group than in the control.NGF expression achieved pinnacle at 3-5 d after fracture.NGFR-TrkA expression was stronger in fracture group than in the control.TrkA achieved pinnacle at 7 d after fracture,at 21 d the expression in cartilage cells was also strong positive.NGFR-p75 expression was stronger in fracture group than in the control and achieved pinnacle at 5-7 d after fracture.At 21 d its expression in fracture group was similar as that in the control.Conclusion： NGF and it＇s receptor p75 and TrkA play important role in the restoring and rebuilding of bone scab during fracture healing.

关 键 词：神经生长因子 受体 骨折愈合 免疫组化 

分 类 号：R782.4[医药卫生—口腔医学]