转录因子T-bet和GATA-3在口腔扁平苔藓患者外周血单核细胞中的表达  

Expression of T cell-specific transcription factors T-bet and GATA-3 in oral lichen planus

作　　者：刘夏斐[1,2] 曾娟[1,2] 赵玉萍[1,2] 范媛[1,2] 许隽永[1,2] 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院黏膜科,210029

出　　处：《实用口腔医学杂志》2011年第4期544-548,共5页Journal of Practical Stomatology

基　　金：南京医科大学科技发展基金面上项目(编号:08NMUM058)

摘　　要：目的:探讨转录因子T-bet和GATA-3在口腔扁平苔藓(OLP)患者外周血单核细胞(PBMCs)中的表达及意义。方法:通过密度梯度离心法分离充血糜烂型及光滑型OLP患者和对照组受试者PBMCs,采用RT-PCR和Western blot法分别检测PBMCs中T-bet、GATA-3 mRNA和蛋白的表达。结果:充血糜烂型、光滑型OLP和对照组外周血单个核细胞中T-bet mRNA表达的相对光密度值分别为0.12±0.04、0.34±0.06和0.57±0.07,T-bet蛋白表达的光吸收度值分别为0.11±0.02、0.40±0.05和0.78±0.07,组间比较差异均有统计学意义(P<0.01);GATA-3 mRNA表达分别为0.83±0.09、0.54±0.07和0.26±0.07,GATA-3蛋白表达分别为0.83±0.060、.55±0.05和0.16±0.03,组间比较差异均有统计学意义(P<0.01)。结论:转录因子T-bet/GATA-3表达失衡是OLP免疫失衡的重要原因之一。Objective： To investigate the expression of the T cell-specific transcription factors T-bet and GATA-3 in peripheral blood mononuclear cells（PBMCs） of patients with oral lichen planus（OLP）.Methods： PBMCs were isolated by density gradient centrifugation from OLP patients with reticular OLP or erythematous/erosive OLP and health controls respectively.Expressions of T-bet and GATA-3 mRNA and protein in the PBMCs were detected by RT-PCR and Western blot respectively.Results： The expressions of T-bet mRNA（relative optical density） in control,reticular OLP and erythematous/erosive OLP groups were 0.57±0.07,0.34±0.06 and 0.12±0.04（P0.01）,the levels of T-bet protein expressions（photoabserption value） were 0.78±0.07,0.40±0.05 and 0.11±0.02（P0.01）,the levels of GATA-3 mRNA expressions were 0.26±0.07,0.54±0.07 and 0.83±0.09（P0.01）,GATA-3 protein expressions were 0.16±0.03,0.55±0.05 and 0.83±0.06,respectively（P0.01）.Conclusion： Imbalance of transcription factors T-bet and GATA-3 may be one of the key factors in immune dysregulation in patients with OLP.

关 键 词：口腔扁平苔藓 转录因子 T淋巴细胞 

分 类 号：R781.5[医药卫生—口腔医学]