LPS刺激神经胶质细胞条件培养上清诱导神经元凋亡  

Apoptosis of neuron induced by supernatant of LPS-stimulated mixed glial cell culture

作　　者：辛岗[1] 苏芸 许燕璇[1] 李康生[1] 

机构地区：[1]汕头大学医学院微生物与免疫学教研室,广东515041

出　　处：《脑与神经疾病杂志》2011年第4期241-243,共3页Journal of Brain and Nervous Diseases

基　　金：国家自然科学基金(30771988;81001340);广东省医学科研基金(A2010392);汕头市重点科技计划项目(汕府科[2009]70号)

摘　　要：目的研究经脂多糖(LPS)刺激后神经胶质细胞培养上清对神经元死亡及凋亡的诱导情况。方法体外原代培养小鼠混合神经胶质细胞和神经元细胞,采用LPS刺激神经胶质细胞,24h后收获条件培养上清与神经元细胞共孵育,检测神经元细胞死亡和凋亡的情况。结果 LPS刺激后的神经胶质细胞培养上清对神经元的毒性作用与未刺激组无明显差别(P>0.05);LPS刺激后的神经胶质细胞条件培养上清诱导神经元细胞凋亡明显高于未刺激组(P<0.05)。结论神经胶质细胞LPS刺激后的条件培养上清可以诱导神经元凋亡,但没有导致明显的神经元细胞死亡。Objective To investigate the apoptosis and death of neuron induced by LPS-stimulated mixed glial cell culture.Methods The mixed glial cells and neurons were cultured.After treated by LPS,the supernatant of cultured mixed glial cells were added into the neurons＇ culture.The death and apoptosis of neurons were detected.Results Compared with the control,the neuron cells could be induced to be apoptosis by the supernatant of LPS-induced mixed glial cell culture（P0.05）,but the death of neuron cells was not so obvious（P0.05）.Conclusion The supernatant of LPS-induced mixed glial cell culture could induce the apoptosis of neuron.

关 键 词：神经元 神经胶质细胞 细胞毒 凋亡 脂多糖 

分 类 号：R741[医药卫生—神经病学与精神病学]