EGF上调hMena的表达促进人胶质瘤细胞迁移能力的体外研究  

Enhancement of Glioma Cell Migration in Vitro by EGF-induced Up-regulation of hMena

作　　者：董雪涛[1] 杨学军[1] 李瑜[1] 于圣平[1] 张斌[1] 王维[1] 王华民[1] 

机构地区：[1]天津医科大学总医院神经外科,天津300052

出　　处：《中国神经肿瘤杂志》2011年第2期77-81,共5页Chinese Journal of Neuro-Oncology

摘　　要：背景与目的:有研究表明hMena可通过EGF启动细胞内多条信号传导道路,指导细胞迁移、黏附、增殖、分化、凋亡等过程。本研究探讨EGF通过上调hMena的表达对胶质瘤细胞迁移运动能力的影响。方法:Western blot检测经100ng/mLEGF处理的人胶质瘤细胞系LN229细胞hMena的表达情况,观察细胞形态的变化,划痕实验与Transwell检测细胞迁移运动能力。结果:100ng/mL EGF可上调人胶质瘤细胞系LN229细胞中hMena的表达(P<0.05),经EGF处理后LN229细胞伪足明显增多,穿过transwell小孔的细胞数为73.67±6.57,显著高于对照组(38.00±5.51)(P<0.05),划痕损伤后迁移的距离为(106.71±5.49)μm,显著高于对照组细胞迁移距离(69.67±6.12)μm,(P<0.05)。结论:EGF可以上调hMena的表达,并增强细胞的迁移运动能力。BACKGROUND OBJECTIVE： Up-regulation of hMENA induced by EGF has been shown to be involved in the cell biology.In this study,we explored the role of up-regulated hMena by EGF.METHODS： LN229 cells were cultured in vitro and treated with 100ng/mL EGF.Western blot was used to detect the expression of hMena.Wound healing assay and Transwell assay were used to determine the migration ability of LN229 cells after treated with EGF.RESULTS： The expression of hMena in LN229 cells was upregulated and the treated cells formed more lamellipodia after administrating 100ng/mL EGF.The number of cells passing through the membrane of Transwell was 73.67±6.57 in EGF treated group compared to control group（38.00±5.51）（P 0.05）.The migration distance was（106.71±5.49）μm in EGF treated group compared to control group（69.67±6.12）μm（P 0.05）.CONCLUSON： EGF up-regulates hMena expression in glioma cells and enhance migration of tumor cells.

关 键 词：胶质瘤 EGF hMena 迁移 

分 类 号：R739.41[医药卫生—肿瘤]