RSK4基因表达及其对U87细胞增殖的影响  

Expression of RSK4 on the Growth of Human U87 Glioma Cells

作　　者：李田田[1,2] 刘明[1] 魏鉴腾[1,2] 吴宁[1,2] 王翠翠[1,2] 林秀坤[1] 

机构地区：[1]中国科学院海洋研究所生物开放室329室,山东青岛266071 [2]中国科学院研究生院,北京100039

出　　处：《中国神经肿瘤杂志》2011年第2期93-97,共5页Chinese Journal of Neuro-Oncology

摘　　要：背景与目的:RSK4是非生长因子依赖的丝/苏氨酸蛋白激酶,参与多种信号通路。本研究构建了人RSK4基因的真核表达系统,研究其对胶质瘤的调控作用,为阐明RSK4功能奠定基础。方法:采用RT-PCR法从人正常乳腺上皮细胞MCF-10A细胞中扩增获得RSK4基因的编码区,定向克隆到真核表达载体pcDNA3.1(-)上,构建真核表达载体pcDNA3.1(-)/RSK4。脂质体法介导pcDNA3.1(-)/RSK4质粒转染至U87细胞中,RT-PCR和Western blot检测表达情况。采用MTT法检测RSK4基因对U87细胞生长的影响。结果:转染pcDNA3.1(-)/RSK4组U87细胞增殖明显被抑制(P<0.05)。结论:RSK4可以抑制胶质瘤细胞的增殖。BACKGROUND OBJECTIVE： RSK4,a class of growth factor-independent threonine/Serinine kinases,plays an important role in siginal transduction.In this study,we constructed an eukaryotic expression system of RSK4 and investigated the role of RSK4 in the growth regulation of U87 cells.METHODS： Human RSK4 eukaryotic expression vector pcDNA3.1（-）/RSK4 was constructed and transfected into human glioma U87 cells.MTT analysis was performed to determine the effect of RSK4 on the proliferation of U87cells.RESULTS： A significant decrease in the proliferation of U87 cells after transfection with pcDNA3.1（-）/RSK4 was observed compared to that in the control groups.CONCLUSON： RSK4 may act as a inhibitor in the proliferation of U87 cells.

关 键 词：RSK4基因 真核表达 载体构建 基因转染 

分 类 号：R739.41[医药卫生—肿瘤]