尾加压素Ⅱ在野百合碱诱导肺动脉高压大鼠中的表达  

Expression of urotensin Ⅱ in sprague-dawley rat with monocrotaline-induced pulmonary hypertension

作　　者：林伟[1] 吴昊[1] 王毅[1] 

机构地区：[1]温州市第三人民医院心血管内科,325000

出　　处：《国际呼吸杂志》2011年第15期1145-1148,共4页International Journal of Respiration

基　　金：温州市科技计划项目（2007A012）

摘　　要：目的通过皮下注射野百合碱诱导Sprague-Dawley（SD）大鼠建立肺动脉高压模型，并探讨尾加压素Ⅱ在此肺动脉高压模型中的表达。方法30只SD大鼠随机分为野百合碱组和正常对照组，每组15只。野百合碱组一次性皮下注射60mg／kg野百合碱，正常对照组以皮下注射同体积生理盐水作对照。给药3周后通过右心导管术检测各组大鼠肺动脉压力参数数值；同时收集各组大鼠血浆及肺组织标本，运用放射免疫法检测血浆中尾加压素Ⅱ含量变化，免疫组织化学法观察肺细小动脉表面尾加压素Ⅱ变化，并进行图像分析。结果野百合碱组大鼠肺动脉各压力参数均较正常对照组有显著升高（P〈0．01），提示野百合碱组大鼠肺动脉高压模型建立成功；另野百合碱组大鼠血浆中尾加压素Ⅱ含量较对照组显著升高（P〈0．01）；其肺细小动脉表面尾加压素Ⅱ蛋白的平均吸光度值也显著高于对照组（P〈0．01），且随着肺动脉分支下移，尾加压素Ⅱ水平逐渐升高。结论肺动脉高压大鼠的血浆及肺小动脉表面尾加压素Ⅱ蛋白含量均显著升高，提示尾加压素Ⅱ可能参与肺动脉高压形成。Objective To study the expression of urotensin Ⅱ （U Ⅱ） in plasma and pulmonary artery of SD rats with monocrotaline（MCT）-induced pulmonary hypertension. Methods Totally 30 male SD rats were divided into the MCT and control group, with 15 rats in each group. Rat model of pulmonary hypertension were established by a single subcutaneous injection of 60 mg/kg MCT, the same volume of saline was used in control group instead of MCT. The rats were killed and the blood and lungs were taken at the end of the third week respectively after the models were established. The expression of UⅡ in plasma was measured by radioimmunoassay, while the expression of UⅡ protein in pulmonary artery tissues was observed by immunohistochemistry and the image was analyzed. Results UⅡ concentrations in plasma were highly increased in rats with MCT-induced pulmonary hypertension than those of the controls（ P〈0.01）, while immunohistochemistry tests also showed the average value of integral light density of UⅡ protein in pulmonary artery tissues were significantly higher than those of the controls（ P〈0, 01）. Furthermore, there were regional differences in the efficacy of UⅡ , with a progressive increase in the maximum contraction from proximal to distal pulmonary artery. Conclusions Concentration of UⅡ in plasma and expression intensity in pulmonary arteries were highly increased in SD rat with MCT-induced pulmonary hypertension, suggesting its potential role in the process of pulmonary hypertension.

关 键 词：尾加压素Ⅱ 野百合碱 肺动脉高压 免疫组织化学 SD大鼠 

分 类 号：R363[医药卫生—病理学]