黄芪多糖和丁酸钠联合用药对K562细胞胎儿血红蛋白合成的作用  

Combined Use of Astragalus Polysaccharide with Sodium Butyrate in Induction of Fetal Hemoglobin Synthesis on K562 Cells

作　　者：曹祥[1] 钱新华[1] 徐梅佳[1] 陈佳[1] 郭丽珊[1] 

机构地区：[1]南方医科大学南方医院新生儿科,广州510515

出　　处：《实用儿科临床杂志》2011年第15期1164-1166,1180,共4页Journal of Applied Clinical Pediatrics

基　　金：广东省自然科学基金(7005146)

摘　　要：目的探讨黄芪多糖(APS)和丁酸钠(NaB)联合用药对人K562细胞胎儿血红蛋白(HbF)合成的作用,为临床联合用药治疗β-珠蛋白生成障碍性贫血(β-地贫)提供实验依据。方法以K562细胞为模型,低剂量APS和NaB联合用药诱导的细胞为实验组,低剂量APS、NaB单药和常规剂量NaB单药诱导的细胞分别为阳性对照组1、2、3,未加药组细胞为对照组4,采用联苯胺染色和Western blot技术分析药物作用K562细胞96 h后红系分化和HbF表达。结果 1.实验组对细胞生长的抑制作用显著弱于对照组3(P<0.05)。2.APS和NaB联合作用K562细胞的最佳剂量组合为APS2.50 g.L-1+NaB 250μmol.L-1。3.联苯胺染色结果显示实验组联苯胺染色阳性率于48 h显著升高,96 h达高峰,与各对照组比较差异均有统计学意义(F=966.630,P<0.05),作用可维持至144 h。4.Western blot结果显示实验组和对照组3诱导K562细胞后HbF合成分别增加至对照组4的(1.82±0.16)倍和(1.57±0.08)倍(F=26.569,P<0.05),实验组HbF表达水平显著高于对照组3(P<0.05)。结论 APS和NaB低剂量联合用药诱导HbF表达增强,作用维持时间长,细胞毒性低,有望成为β-地贫的一种新的治疗方案。Objective To provide an experimental basis for clinical combination therapy of β-thalassemia through investigating the effects of combined use of low-dose astragalus polysaccharide（APS） and sodium butyrate（NaB） in the induction of fetal hemoglobin（HbF） synthesis on K562 cells.Methods K562 cells were chosen as the cell model,cells treated with low-dose APS combined with NaB were taken as the experimental group,while low-dose APS,low-dose NaB and regular-dose NaB were set up as the positive control group 1,2,3 and untreated cells as the control group 4.Benzidine staining and Western blot were used to analyze erythroid differentiation and HbF expression in K562 cells after getting treated with drugs for 96 h.Results 1.The K562 cell inhibiting effect,treated with combined use of low-dose APS and NaB,was significantly weaker than the positive control group 3（P0.05）.2.The optimal combination for which APS and NaB could display to effect K562 cells was 2.50 g·L-1 APS with 250 μmol·L-1 NaB.3.The results of benzidine staining displayed that the percentages of benzidine-positive cells in the experimental group significantly increased from 48 h to 144 h,reaching the peak at 96 h,which were significantly higher than each control group（F=966.630,P0.05）.4.When compared with control group 4,the HbF expression levels increased to（1.82±0.16） and（1.57±0.08） times higher in experimental group and positive control group 3,respectively（F=26.569,P0.05）;The HbF expression levels were much higher in experimental group than in positive control group 3（P0.05）.Conclusion Low-dose APS combined with NaB can induce HbF synthesis more effectively,more lasting and can reduce cytotoxicity,which has the potential to be a new therapeutic schedule for β-thalassemia.

关 键 词：Β-珠蛋白生成障碍性贫血 K562细胞 黄芪多糖 丁酸钠 联合用药 

分 类 号：R725.5[医药卫生—儿科]