NO-1886对高脂/高糖/高胆固醇饲养的小型猪组织中肿瘤坏死因子α和白细胞介素1β蛋白表达的影响  

Effects of NO-1886 on the Protein Expressions of TNF-α and IL-1β in High Fat/High Sucrose/High Cholesterol Diet Fed Miniature Pigs

作　　者：蔡曼波[1] 李建军 胡丽[1] 何平平[1] 周钰娟[1] 尹卫东[3] 

机构地区：[1]南华大学护理学院,湖南省衡阳市421001 [2]南华大学附属第二医院,湖南省衡阳市421001 [3]南华大学心血管研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2011年第9期741-746,共6页Chinese Journal of Arteriosclerosis

摘　　要：目的为了探讨脂蛋白酯酶活化剂NO-1886对高糖/高脂/高胆固醇饲养的小型猪组织中肿瘤坏死因子α及白细胞介素1β蛋白表达的影响。方法选择15只雄性巴马小型猪,按体重随机分为3组,分别饲以基础猪饲料(正常对照组),高脂/高糖/高胆固醇饲料(三高组)和高脂/高糖/高胆固醇饲料里加1.0%NO-1886(治疗组)。每个月末从巴马猪眶静脉窦采集血样,检测血浆甘油三酯和游离脂肪酸的浓度。5个月后处死动物,用透射电镜检测脂质在小型猪肝脏组织中的异位蓄积情况,免疫印迹技术检测肿瘤坏死因子α和白细胞介素1β在肝脏和脂肪组织中的蛋白表达。结果高脂/高糖/高胆固醇饮食诱导广西巴马小型猪发生了脂代谢的紊乱,引起小型猪空腹血浆甘油三酯和游离脂肪酸水平升高,诱发肝脏组织脂质异位蓄积,诱导广西巴马小型猪肝脏和脂肪等组织中炎症因子肿瘤坏死因子α和白细胞介素1β的蛋白表达增加;而在高脂/高糖/高胆固醇饲料中添加1.0%NO-1886后,NO-1886明显降低血浆甘油三酯和游离脂肪酸水平,抑制肝脏组织脂质异位蓄积,并降低肝脏和脂肪等组织中炎症因子肿瘤坏死因子α及白细胞介素1β的蛋白表达。结论 NO-1886能明显抑制脂质在肝脏组织中的异位蓄积,改善脂代谢,降低高脂/高糖/高胆固醇饲养的小型猪组织中肿瘤坏死因子α及白细胞介素1β的蛋白表达,进而抑制动脉粥样硬化的发生发展。Aim To investigate the role of lipoprotein lipase （LPL） activator NO-1886 on the protein expressions of tumor necrosis factor-α（TNF-α） and IL-1β in high-fat/high-sucrose/high-cholesterol diet （HFSCD） fed miniature pigs. Methods Male, fifteen Bama-miniature pigs were randomized into three groups： control group, high-fat/highsucrose/high-cholesterol （three high） group and NO-1886 treated （HFSCD ＋ NO-1886） group. Blood samples for plasma parameters including plasma TG and FFA were withdrawn from the orbital sinus of the animals at the end of each month following an overnight fast. The pigs were sacrificed at the end of month 5. The degree of lipid deposition in liver was examined by transmission electron microscope. The protein levels of TNF-α and IL-lbeta in liver and fat tissues were measured by western blotting. Results The lipid metabolic disorder was induced in Guangxi Bama-miniature pigs by feeding high fat/high sucrose/high cholesterol diet, levels of plasma fasting TG and FFA were increased, which induced ectopic visceral lipid deposition in liver tissue and increased the protein levels of TNF-α and IL-1βin liver and fat tissues. While NO-1886 decreased the level of TG and FFA, ameliorated visceral fat deposition and reduced the protein expres-sions of TNF-oL and IL-lbeta in liver and fat tissues. Conclusions NO-1886 may significantly meliorate ectopic visceral lipid deposition, ameliorated lipid metabolism, decrease the protein expressions of TNF-α and IL-1β in tissues of high-fat/high-sucrose/highcholesterol diet fed miniature pigs, and inhibit the progress of atherosclerosis .

关 键 词：NO-1886 脂蛋白酯酶 肿瘤坏死因子Α 白细胞介素1Β 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]