骨髓间质细胞和非骨髓间质细胞源细胞移植后机体免疫反应差异的比较  

Difference of immune response to transplantation of BMSCs and non-BMSCs-derived cells in animals

作　　者：刘芳[1,2,3] 刘大伟[2,3] 范新娟[2,3] 唐芳[2,3] 孙丽霞[2,3] 赵国强[2,3] 

机构地区：[1]南方医科大学附属南方医院肿瘤中心,广东广州510515 [2]中山大学附属第一医院病理科,广东广州510080 [3]中山大学中山医学院病理学教研室,广东广州510080

出　　处：《中国病理生理杂志》2011年第7期1270-1274,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30170473);广东省自然科学基金资助项目(No.04009326)

摘　　要：目的:比较骨髓间质细胞(BMSCs)和非骨髓间质细胞源细胞移植后体内的免疫反应特征。方法:选用SD大鼠和新西兰大白兔作为细胞移植的受体动物,分别将大鼠BMSCs和肝细胞株BRL经静脉和皮下植入动物体内,通过分析外周血淋巴细胞和单核细胞数目变化及皮下移植位点组织病理学特征,揭示2种细胞移植后体内的免疫反应差异。结果:无论在同种异体移植组或异种交叉移植组,BMSCs所引起的淋巴细胞增殖强度均低于BRL移植组,动物对BMSCs移植的免疫反应较为迟钝;而单核细胞的增殖能力则是BMSCs移植组高于BRL移植组。组织病理学结果显示,BMSCs移植组未在移植位点附近观察到明显的细胞坏死及炎症细胞浸润。结论:由BMSCs引发的免疫反应强度低于BRL,在细胞移植中抵御免疫排斥的能力要优于BRL。AIM： To analyze the characteristics of immune response in the animals after transplantation of bone marrow stromal cells（BMSCs） or non-BMSCs-derived cells.METHODS： Rat BMSCs and Buffalo rat liver（BRL） cells were transplanted into SD rats and New Zealand rabbits by intravenous and subcutaneous injections.The differences of immune response in the animals received these two types of cells were evaluated by comparative analysis of the lymphocytes and monocytes in peripheral blood as well as the histopathological characteristics of the subcutaneous transplantation sites.RESULTS： The increase intensity of the lymphocyte number in BMSCs group was lower than that in BRL group,regardless of allograft or xenograft.The immune reaction of the animals to BMSCs transplantation was a little slower.It was obviously dissimilar that the increase intensity of monocyte number in BMSCs group was higher than that in BRL group.Pathological cutaneous sections showed that neither obvious necrosis nor inflammatory cells was observed in the vicinity of BMSCs transplantation sites.CONCLUSION： The intensity of immune response induced by BMSCs is lower than that by BRL cells.The anti-rejection ability of BMSCs in cell transplantation is better than that of BRL cells.

关 键 词：骨髓间质细胞 细胞移植 免疫反应 

分 类 号：R363[医药卫生—病理学]