PPARγ激动剂干预对激活星形胶质细胞Jagged1的影响  

Effect of PPARγ agonist on Jagged1 mRNA expression in activated astrocytes

作　　者：曾彦英[1] 陈伟贤[1] 高霞[1] 王峥[1] 顾冰洁[1] 王松[2] 季晓辉[2] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210029 [2]南京医科大学免疫与微生物教研室

出　　处：《江苏医药》2011年第15期1737-1739,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅"135"工程重点学科基金(200131);江苏省高校自然科学研究计划自筹经费项目(08KJD320004)

摘　　要：目的观察过氧化物酶体增殖物激活受体γ(PPARγ)激动剂吡格列酮干预对激活星形胶质细胞产生Jagged1的影响。方法采用脂多糖(LPS)刺激纯化的星形胶质细胞,将不同梯度浓度吡格列酮(0.01,0.1,1.0,10.0μm)和转化生长因子β1(10 ng/ml)共刺激星形胶质细胞6 h,提取细胞RNA。结果吡格列酮呈浓度依存性抑制LPS激活星形胶质细胞中Jagged1的mRNA表达(P<0.05)。结论吡格列酮抑制星形胶质细胞Jagged1的产生,Jagged1/Notch通路的激活可能随之减弱,有利于多发性硬化的髓鞘修复。Objective To investigate the effect of peroxisome proliferator-activated receptor-gamma（PPARγ） agonist on Jagged1 mRNA expression in activated astrocytes.Methods Astrocyte activated by LPS was treated by pioglitazone with different concentrations（0.01,0.1,1.0,10.0 μm）and TGF-β1（10 ng/ml） for 6 hours.Then the Jagged1 mRNA was extracted and the expression of Jagged1 mRNA was evaluated with fluorescent quantixative RT-PCR.Results Pioglitazone inhibited the Jagged1 mRNA expression of astrocytes in a concentration-dependent manner（P〈0.05）.Conclusion Pioglitazone inhibits Jagged1 mRNA expression of astrocytes,which leads to reducing the activation of Jagged1/Notch1 pathway and facilitating the repair of myelin sheath of multiple sclerosis.

关 键 词：过氧化物酶体增殖物激活受体Γ 星形胶质细胞 Jaggedl基因 转化生长因子Β1 

分 类 号：R741[医药卫生—神经病学与精神病学]