绿色荧光蛋白转染标记腺样囊性癌生长的裸鼠模型  

Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma

作　　者：陈正岗[1,2] 董作青[1,2] 张东[1,2] 周成军[3] 孙善珍[4] 刘少华[1,2] 魏奉才[1,2] 

机构地区：[1]山东大学齐鲁医院口腔颌面外科 [2]山东大学口腔医学研究所,济南250012 [3]山东大学第二医院病理科,济南250033 [4]山东大学口腔医院病理科,济南250012

出　　处：《华西口腔医学杂志》2011年第4期424-428,共5页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30672339);教育部留学回国人员科研启动基金资助项目(2007)

摘　　要：目的建立能够实时监测、连续动态观察腺样囊性癌生长、转移的裸鼠模型。方法用逆转录病毒载体pLEGFP-N1感染腺样囊性癌细胞系ACCM并筛选出ACCM-GFP稳定细胞株,并比较ACCM和ACCM-GFP细胞的增殖特性和形态特点;用ACCM-GFP细胞种植法建立裸鼠舌癌模型,对移植瘤的生长进行实时荧光成像观察,并与ACCM肿瘤进行组织学比较。结果 ACCM-GFP细胞可长期稳定表达GFP;ACCM和ACCM-GFP细胞的增殖特性和形态无差异,体内成瘤的组织学表现无差异;通过活体荧光成像系统可实时监测肿瘤的生长。结论 ACCM-GFP裸鼠荧光肿瘤模型可以长期无创、实时、动态观察肿瘤的生长变化,是进行腺样囊性癌基础和临床研究的理想肿瘤模型。Objective To establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.Methods Human adenoid cystic carcinoma cells,ACCM cell line,were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells.Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells.Nude mice lingual carcinoma model was set up with ACCM-GFP cells injection and real time observation with fluorescence imaging on ACCM-GFP tumors was performed subsequently.Histological assay was analyzed for ACCM and ACCM-GFP tumors as well.Results ACCM-GFP cells were able to express GFP stably in the long term.ACCM and ACCM-GFP cells showed no significant difference in cell proliferation and morphology,and no significant difference of histological characteristics in vivo could be found between ACCM and ACCM-GFP tumors.Tumor development could be monitored in real time with fluorescence imaging system in vivo.Conclusion GFP-expressing ACCM tumor model can be applied to detect and observe its development in the long term in a noninvasive,real time and dynamic way.It is also a kind of ideal in vivo mouse model for adenoid cystic carcinoma research.

关 键 词：腺样囊性癌 绿色荧光蛋白 肿瘤模型 动物模型 

分 类 号：R739.87[医药卫生—肿瘤]